用于确定92种肺炎球菌血清型的单步多重PCR检测法
Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.
作者信息
Marimón José M, Ercibengoa María, Santacatterina Erica, Alonso Marta, Pérez-Trallero Emilio
机构信息
Microbiology Department, Hospital Universitario Donostia-Instituto de Investigación Sanitaria Biodonostia, San Sebastián, Spain Biomedical Research Center Network for Respiratory Diseases (CIBERES), Madrid, Spain
Biomedical Research Center Network for Respiratory Diseases (CIBERES), Madrid, Spain.
出版信息
J Clin Microbiol. 2016 Aug;54(8):2197-200. doi: 10.1128/JCM.01156-16. Epub 2016 Jun 8.
Abstract
For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.
摘要
对于肺炎球菌疾病监测，需要能够确定所有血清型的简单且经济高效的方法。将单管多重PCR与荧光标记引物相结合，随后使用自动荧光毛细管电泳进行扩增子分析，成功确定了92株参考菌株和297株近期收集的临床菌株的每种血清型。
相似文献
1
Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.用于确定92种肺炎球菌血清型的单步多重PCR检测法
J Clin Microbiol. 2016 Aug;54(8):2197-200. doi: 10.1128/JCM.01156-16. Epub 2016 Jun 8.
2
Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae.基于聚合酶链反应的肺炎链球菌血清型测定方法的评估
Trop Biomed. 2013 Jun;30(2):338-44.
3
Practical Prediction of Ten Common Streptococcus pneumoniae Serotypes/Serogroups in One PCR Reaction by Multiplex Ligation-Dependent Probe Amplification and Melting Curve (MLPA-MC) Assay in Shenzhen, China.在中国深圳，通过多重连接依赖探针扩增和熔解曲线（MLPA-MC）分析法在一次聚合酶链反应中对十种常见肺炎链球菌血清型/血清群进行实际预测。
PLoS One. 2015 Jul 7;10(7):e0130664. doi: 10.1371/journal.pone.0130664. eCollection 2015.
4
Using a practical molecular capsular serotype prediction strategy to investigate Streptococcus pneumoniae serotype distribution and antimicrobial resistance in Chinese local hospitalized children.采用实用的分子荚膜血清型预测策略研究中国当地住院儿童肺炎链球菌的血清型分布及耐药性。
BMC Pediatr. 2016 Apr 26;16:53. doi: 10.1186/s12887-016-0589-7.
5
Customized sequential multiplex PCR for accurate and early determination of invasive pneumococcal serotypes found in India.定制的序列多重PCR用于准确早期测定印度发现的侵袭性肺炎球菌血清型。
J Microbiol Methods. 2016 Nov;130:133-135. doi: 10.1016/j.mimet.2016.09.007. Epub 2016 Sep 10.
6
The Relevance of a Novel Quantitative Assay to Detect up to 40 Major Streptococcus pneumoniae Serotypes Directly in Clinical Nasopharyngeal and Blood Specimens.一种新型定量检测方法在临床鼻咽和血液标本中直接检测多达40种主要肺炎链球菌血清型的相关性。
PLoS One. 2016 Mar 17;11(3):e0151428. doi: 10.1371/journal.pone.0151428. eCollection 2016.
7
Evaluation of Modified Sequential Multiplex PCR for Streptococcus pneumoniae Serotyping.改良序贯多重 PCR 法用于肺炎链球菌血清分型的评价。
Jpn J Infect Dis. 2019 Jul 24;72(4):224-227. doi: 10.7883/yoken.JJID.2018.422. Epub 2019 Feb 28.
8
Serotyping of pneumococci: evaluation of the genetic approach and performance with clinical samples.肺炎球菌的血清分型：基因方法的评估及其在临床样本中的表现
Diagn Microbiol Infect Dis. 2014 Dec;80(4):274-7. doi: 10.1016/j.diagmicrobio.2014.08.013. Epub 2014 Sep 2.
9
PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18.基于PCR的肺炎链球菌培养阴性标本血清分型：用于检测18血清群内血清型的新型引物
J Clin Microbiol. 2016 Aug;54(8):2178-81. doi: 10.1128/JCM.00419-16. Epub 2016 Jun 1.
10
Evaluation of pneumococcal serotyping by multiplex PCR and quellung reactions.通过多重聚合酶链反应和荚膜肿胀反应进行肺炎球菌血清分型评估。
J Clin Microbiol. 2013 Dec;51(12):4193-5. doi: 10.1128/JCM.01876-13. Epub 2013 Sep 11.
引用本文的文献
1
Persistence of Vaccine Serotype Carriage and Differences in Pneumococcal Carriage by Laboratory Method and Sample Type in Indigenous Individuals in the Southwest United States.美国西南部原住民中疫苗血清型携带情况的持续存在以及通过实验室方法和样本类型检测肺炎球菌携带情况的差异
J Infect Dis. 2025 Jul 11;231(6):e1045-e1056. doi: 10.1093/infdis/jiaf091.
2
Pneumococcal Serotypes Associated with Community-Acquired Pneumonia Hospitalizations in Adults in Spain, 2016-2020: The CAPA Study.2016 - 2020年西班牙成人社区获得性肺炎住院相关的肺炎球菌血清型：CAPA研究
Microorganisms. 2023 Nov 16;11(11):2781. doi: 10.3390/microorganisms11112781.
3
Impact of the progressive uptake of pneumococcal conjugate vaccines on the epidemiology and antimicrobial resistance of invasive pneumococcal disease in Gipuzkoa, northern Spain, 1998-2022.西班牙北部吉普斯夸省（Gipuzkoa）1998-2022 年肺炎球菌结合疫苗逐步推广对侵袭性肺炎球菌病的流行病学和抗菌药物耐药性的影响。
Front Public Health. 2023 Aug 31;11:1238502. doi: 10.3389/fpubh.2023.1238502. eCollection 2023.
4
Co-infection with dual Streptococcus pneumoniae serotypes as a cause of pediatric bacterial meningitis in Iran: a multi-center cross-sectional study.伊朗儿童细菌性脑膜炎双型肺炎链球菌共感染的病因：一项多中心横断面研究。
BMC Infect Dis. 2022 Jul 18;22(1):625. doi: 10.1186/s12879-022-07606-w.
5
Molecular Detection and Serotyping of in Children with Suspected Meningitis in Northeast Iran.伊朗东北部疑似脑膜炎患儿中[具体物质未明确]的分子检测与血清分型
Iran J Med Sci. 2020 Mar;45(2):125-133. doi: 10.30476/IJMS.2019.45423.
6
Identification of Pneumococcal Serotypes by PCR-Restriction Fragment Length Polymorphism.通过聚合酶链反应-限制性片段长度多态性鉴定肺炎球菌血清型
Diagnostics (Basel). 2019 Nov 18;9(4):196. doi: 10.3390/diagnostics9040196.
7
Utility of MALDI-TOF MS as a new tool for Streptococcus pneumoniae serotyping.基质辅助激光解吸电离飞行时间质谱技术（MALDI-TOF MS）作为一种新型工具在肺炎链球菌血清分型中的应用。
PLoS One. 2019 Feb 12;14(2):e0212022. doi: 10.1371/journal.pone.0212022. eCollection 2019.
8
Changes in the serotype distribution of Streptococcus pneumoniae causing otitis media after PCV13 introduction in Spain.13 价肺炎球菌结合疫苗（PCV13）引入西班牙后引起中耳炎的肺炎链球菌血清型分布变化。
PLoS One. 2018 Dec 18;13(12):e0209048. doi: 10.1371/journal.pone.0209048. eCollection 2018.
本文引用的文献
1
The impact of private use of PCV7 in 2009 and 2010 on serotypes and antimicrobial resistance of Streptococcus pneumoniae carried by young children in Portugal: Comparison with data obtained since 1996 generating a 15-year study prior to PCV13 introduction.2009年和2010年葡萄牙私人使用7价肺炎球菌结合疫苗（PCV7）对幼儿携带的肺炎链球菌血清型及耐药性的影响：与1996年以来获得的数据进行比较，形成了在13价肺炎球菌结合疫苗（PCV13）引入之前的15年研究。
Vaccine. 2016 Mar 29;34(14):1648-56. doi: 10.1016/j.vaccine.2016.02.045. Epub 2016 Feb 23.
2
The Effect of 13-Valent Pneumococcal Conjugate Vaccine on the Serotype Distribution and Antibiotic Resistance Profiles in Children With Invasive Pneumococcal Disease.13 价肺炎球菌结合疫苗对儿童侵袭性肺炎球菌病的血清型分布和抗生素耐药谱的影响。
J Pediatric Infect Dis Soc. 2017 Sep 1;6(3):253-259. doi: 10.1093/jpids/piw005.
3
Pneumococcal Capsules and Their Types: Past, Present, and Future.肺炎球菌荚膜及其类型：过去、现在与未来
Clin Microbiol Rev. 2015 Jul;28(3):871-99. doi: 10.1128/CMR.00024-15.
4
Detection and prediction of Streptococcus pneumoniae serotypes directly from nasopharyngeal swabs using PCR.使用聚合酶链反应（PCR）直接从鼻咽拭子中检测和预测肺炎链球菌血清型
J Med Microbiol. 2015 Aug;64(8):836-844. doi: 10.1099/jmm.0.000097. Epub 2015 Jun 11.
5
Serotyping of pneumococci: evaluation of the genetic approach and performance with clinical samples.肺炎球菌的血清分型：基因方法的评估及其在临床样本中的表现
Diagn Microbiol Infect Dis. 2014 Dec;80(4):274-7. doi: 10.1016/j.diagmicrobio.2014.08.013. Epub 2014 Sep 2.
6
European enhanced surveillance of invasive pneumococcal disease in 2010: data from 26 European countries in the post-heptavalent conjugate vaccine era.2010年欧洲侵袭性肺炎球菌疾病强化监测：来自26个欧洲国家在七价结合疫苗时代后的数据。
Vaccine. 2014 Jun 17;32(29):3644-50. doi: 10.1016/j.vaccine.2014.04.066. Epub 2014 May 2.
7
One-step multiplex PCR assay for detecting Streptococcus pneumoniae serogroups/types covered by 13-valent pneumococcal conjugate vaccine (PCV13).一步法多重 PCR 检测试剂盒用于检测 13 价肺炎球菌结合疫苗（PCV13）涵盖的肺炎链球菌血清型/型别。
PLoS One. 2012;7(12):e50406. doi: 10.1371/journal.pone.0050406. Epub 2012 Dec 4.
8
Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden.用于检测导致高全球疾病负担的 21 种肺炎球菌荚膜血清型的三重实时 PCR 序贯检测法。
J Clin Microbiol. 2013 Feb;51(2):647-52. doi: 10.1128/JCM.02927-12. Epub 2012 Dec 5.
9
Prevention of pneumococcal diseases in the post-seven valent vaccine era: a European perspective.七价肺炎球菌结合疫苗时代之后的肺炎球菌疾病预防：欧洲视角。
BMC Infect Dis. 2012 Sep 7;12:207. doi: 10.1186/1471-2334-12-207.
10
Rapid and easy identification of capsular serotypes of Streptococcus pneumoniae by use of fragment analysis by automated fluorescence-based capillary electrophoresis.应用基于荧光的自动化毛细管电泳片段分析技术快速、简便地鉴定肺炎链球菌荚膜血清型。
J Clin Microbiol. 2012 Nov;50(11):3451-7. doi: 10.1128/JCM.01368-12. Epub 2012 Aug 8.
Zotero 插件