Institut für Anorganische Chemie, RWTH Aachen University, Landoltweg 1, 52056 Aachen (Germany).
Chemistry. 2013 Oct 4;19(41):13865-71. doi: 10.1002/chem.201301515. Epub 2013 Aug 19.
A β-barrel protein hybrid catalyst was prepared by covalently anchoring a Grubbs-Hoveyda type olefin metathesis catalyst at a single accessible cysteine amino acid in the barrel interior of a variant of β-barrel transmembrane protein ferric hydroxamate uptake protein component A (FhuA). Activity of this hybrid catalyst type was demonstrated by ring-opening metathesis polymerization of a 7-oxanorbornene derivative in aqueous solution.
通过在β-桶状跨膜蛋白 ferric hydroxamate uptake protein component A（FhuA）变体的桶内单个可及半胱氨酸氨基酸上共价连接 Grubbs-Hoveyda 型烯烃复分解催化剂，制备了β-桶状蛋白杂化催化剂。该杂化催化剂类型的活性通过在水溶液中环氧化合物开环复分解聚合 7-氧杂降冰片烯衍生物得到证明。
