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Dynamic, electrostatic model for the generation and control of high-energy radical intermediates by a coenzyme B₁₂-dependent enzyme.

作者信息

Chen Zhi-Gang, Ziętek Monika A, Russell Henry J, Tait Shirley, Hay Sam, Jones Alex R, Scrutton Nigel S

机构信息

College of Food and Science Technology, Nanjing Agricultural University, 1 Weigang Road, Nanjing 210095 (P.R. China).

出版信息

Chembiochem. 2013 Sep 2;14(13):1529-33. doi: 10.1002/cbic.201300420. Epub 2013 Aug 19.

DOI:10.1002/cbic.201300420
PMID:23959797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4155860/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/d4ef60e02538/cbic0014-1529-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/0dfae79a0238/cbic0014-1529-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/32ac15062bfd/cbic0014-1529-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/9cbe24d3b9fe/cbic0014-1529-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/0e915dc116e0/cbic0014-1529-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/d4ef60e02538/cbic0014-1529-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/0dfae79a0238/cbic0014-1529-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/32ac15062bfd/cbic0014-1529-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/9cbe24d3b9fe/cbic0014-1529-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/0e915dc116e0/cbic0014-1529-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/818f/4155860/d4ef60e02538/cbic0014-1529-f4.jpg

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本文引用的文献

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FEBS J. 2013 Jul;280(13):2997-3008. doi: 10.1111/febs.12223. Epub 2013 Mar 21.
2
Mutagenesis of a conserved glutamate reveals the contribution of electrostatic energy to adenosylcobalamin co-C bond homolysis in ornithine 4,5-aminomutase and methylmalonyl-CoA mutase.突变一个保守的谷氨酸残基揭示了静电能在鸟氨酸 4,5-氨基甲酰转移酶和甲基丙二酰辅酶 A 变位酶中的腺苷钴胺素共 C 键均裂中的贡献。
Biochemistry. 2013 Feb 5;52(5):878-88. doi: 10.1021/bi3012719. Epub 2013 Jan 24.
3
一种依赖维生素B12的光感受器蛋白的光化学机制。
Nat Commun. 2015 Aug 12;6:7907. doi: 10.1038/ncomms8907.
4
Glutamate 338 is an electrostatic facilitator of C-Co bond breakage in a dynamic/electrostatic model of catalysis by ornithine aminomutase.在鸟氨酸氨基变位酶催化的动态/静电模型中，谷氨酸338是C-Co键断裂的静电促进剂。
FEBS J. 2015 Apr;282(7):1242-55. doi: 10.1111/febs.13215. Epub 2015 Feb 12.
5
A conformational sampling model for radical catalysis in pyridoxal phosphate- and cobalamin-dependent enzymes.磷酸吡哆醛和钴胺素依赖性酶中自由基催化的构象抽样模型
J Biol Chem. 2014 Dec 5;289(49):34161-74. doi: 10.1074/jbc.M114.590471. Epub 2014 Sep 11.
Experimental study of hydrogen bonding potentially stabilizing the 5'-deoxyadenosyl radical from coenzyme B12.
辅酶 B12 中 5′-脱氧腺嘌呤核苷自由基氢键潜在稳定作用的实验研究。
Chemistry. 2012 Dec 7;18(50):16114-22. doi: 10.1002/chem.201201840. Epub 2012 Oct 18.
4
Hydrogen bonds guide the short-lived 5'-deoxyadenosyl radical to the place of action.氢键将短寿命的5'-脱氧腺苷自由基引导至作用位点。
Angew Chem Int Ed Engl. 2012 Oct 1;51(40):9974-6. doi: 10.1002/anie.201205299. Epub 2012 Sep 3.
5
Protein motions are coupled to the reaction chemistry in coenzyme B12-dependent ethanolamine ammonia lyase.在依赖辅酶B12的乙醇胺氨裂解酶中，蛋白质运动与反应化学过程相耦合。
Angew Chem Int Ed Engl. 2012 Sep 10;51(37):9306-10. doi: 10.1002/anie.201202502. Epub 2012 Aug 15.
6
Ultrafast infrared spectral fingerprints of vitamin B12 and related cobalamins.维生素 B12 及其相关钴胺素的超快红外光谱指纹。
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Large-scale domain conformational change is coupled to the activation of the Co-C bond in the B12-dependent enzyme ornithine 4,5-aminomutase: a computational study.大规模的结构域构象变化与 B12 依赖性酶鸟氨酸 4,5-氨基转移酶中 Co-C 键的激活相偶联：一项计算研究。
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The elusive 5'-deoxyadenosyl radical in coenzyme-B12-mediated reactions.辅酶 B12 介导反应中的 elusive 5'-脱氧腺嘌呤核苷自由基。
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