使用血清型特异性马鼻炎B病毒（ERBV）酶联免疫吸附试验对澳大利亚断奶马进行马鼻炎B病毒（ERBV）血清流行率研究。

Seroprevalence study of Equine rhinitis B virus (ERBV) in Australian weanling horses using serotype-specific ERBV enzyme-linked immunosorbent assays.

作者信息

Horsington Jacquelyn, Hartley Carol A, Gilkerson James R

机构信息

1Carol A. Hartley, Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3052, Australia.

出版信息

J Vet Diagn Invest. 2013 Sep;25(5):641-4. doi: 10.1177/1040638713500783. Epub 2013 Aug 19.

DOI:10.1177/1040638713500783

PMID:23960171

Abstract

Respiratory infections are a major burden in the performance horse industry. Equine rhinitis B virus (ERBV) has been isolated from horses displaying clinical respiratory disease, and ERBV-neutralizing antibodies have been detected in 50-80% of horses in reported surveys. Current ERBV isolation and detection methods may underestimate the number of ERBV-positive animals and do not identify multiple serotype infections. The aim of the current study was to develop a serotyping ERBV antibody-detection enzyme-linked immunosorbent assay (ELISA) and examine the seroprevalence of ERBV in a group of Australian weanling horses. ELISAs with high sensitivity and specificity were developed. The seroprevalence of ERBV in the weanling horses was high (74-86%); ERBV-3 antibodies were most prevalent (58-62%) and ERBV-2 antibodies were least prevalent (10-16%). Many horses were seropositive to 2 or more serotypes. All 3 serotypes of ERBV were detected, and concurrent positivity to multiple serotypes was common.

摘要

呼吸道感染是竞技马匹行业的一个主要负担。已从出现临床呼吸道疾病的马匹中分离出马鼻疽B病毒（ERBV），并且在报告的调查中，50%-80%的马匹检测到了ERBV中和抗体。目前的ERBV分离和检测方法可能会低估ERBV阳性动物的数量，并且无法识别多种血清型感染。本研究的目的是开发一种用于ERBV血清分型的抗体检测酶联免疫吸附测定（ELISA），并检测一组澳大利亚断奶幼驹中ERBV的血清流行率。开发出了具有高灵敏度和特异性的ELISA。断奶幼驹中ERBV的血清流行率很高（74%-86%）；ERBV-3抗体最为普遍（58%-62%），而ERBV-2抗体最不常见（10%-16%）。许多马匹对2种或更多血清型呈血清阳性。检测到了所有3种血清型的ERBV，同时感染多种血清型的情况很常见。