从对有呼吸道症状的马鼻分泌物进行的 7 年马鼻炎 B 病毒 qPCR 检测中，我们了解到了什么。

What have we learned from 7 years of equine rhinitis B virus qPCR testing in nasal secretions from horses with respiratory signs.

机构信息

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA.

出版信息

Vet Rec. 2021 May;188(10):e26. doi: 10.1002/vetr.26. Epub 2021 Mar 24.

DOI:10.1002/vetr.26

PMID:33759193

Abstract

BACKGROUND

Equine rhinitis B virus (ERBV) has been given little attention by practitioners compared to other respiratory viruses, mainly because of the lack of diagnostic modalities and association with clinical disease. The objective of the study was to determine the frequency of detection of ERBV in nasal secretions from 6568 horses with acute onset of respiratory signs.

METHODS

ERBV-positive qPCR results from nasal secretions submitted to a molecular diagnostic laboratory from 2013 to 2019 were reviewed.

RESULTS

A total of 333 ERBV qPCR-positive samples (5.1%) were detected with increasing yearly frequency since the introduction of the assay in 2013. In comparison, only three of 356 (0.8%) healthy horses tested qPCR-positive for ERBV. Median age for ERBV qPCR-positive horses was 3 years of age, and fever, coughing and nasal discharge were the most common signs reported. Further, co-infections with other respiratory pathogens were reported in 73 (21.9%) of ERBV qPCR-positive samples.

CONCLUSION

ERBV is a commonly detected respiratory virus from nasal secretions of young horses presenting with fever, nasal discharge and coughing.

摘要

背景

与其他呼吸道病毒相比，马鼻炎 B 病毒（ERBV）引起的疾病较少引起临床医生的关注，主要是因为缺乏诊断方法和与临床疾病的关联。本研究的目的是确定在 6568 例急性呼吸道症状的马的鼻腔分泌物中 ERBV 的检出频率。

方法

对 2013 年至 2019 年提交给分子诊断实验室的鼻腔分泌物中 ERBV 阳性 qPCR 结果进行了回顾性分析。

结果

自 2013 年引入该检测方法以来，共检测到 333 份 ERBV qPCR 阳性样本（5.1%），且阳性检出率呈逐年上升趋势。相比之下，仅 356 份健康马的样本中有 3 份（0.8%）为 ERBV qPCR 阳性。ERBV qPCR 阳性马的中位年龄为 3 岁，发热、咳嗽和鼻腔分泌物是最常见的报告症状。此外，在 73 份（21.9%）ERBV qPCR 阳性样本中报告了其他呼吸道病原体的合并感染。