Gherardini Lisa, Gennaro Mariangela, Pizzorusso Tommaso
Institute of Neuroscience, CNR, Pisa 56124, Italy, Institute of Clinical Physiology, CNR, Siena 53100, Italy and.
Institute of Neuroscience, CNR, Pisa 56124, Italy.
Cereb Cortex. 2015 Jan;25(1):202-12. doi: 10.1093/cercor/bht217. Epub 2013 Aug 19.
Ischemic stroke insults may lead to chronic functional limitations that adversely affect patient movements. Partial motor recovery is thought to be sustained by neuronal plasticity, particularly in areas close to the lesion site. It is still unknown if treatments acting exclusively on cortical plasticity of perilesional areas could result in behavioral amelioration. We tested whether enhancing plasticity in the ipsilesional cortex using local injections of chondroitinase ABC (ChABC) could promote recovery of skilled motor function in a focal cortical ischemia of forelimb motor cortex in rats. Using the skilled reaching test, we found that acute and delayed ChABC treatment induced recovery of impaired motor skills in treated rats. vGLUT1, vGLUT2, and vGAT staining indicated that functional recovery after acute ChABC treatment was associated with local plastic modification of the excitatory cortical circuitry positive for VGLUT2. ChABC effects on vGLUT2 staining were present only in rats undergoing behavioral training. Thus, the combination of treatments targeting the CSPG component of the extracellular matrix in perilesional areas and rehabilitation could be sufficient to enhance functional recovery from a focal stroke.
缺血性中风损伤可能导致慢性功能受限，对患者的运动产生不利影响。部分运动恢复被认为是由神经元可塑性维持的，尤其是在病变部位附近的区域。仅作用于病灶周围区域皮质可塑性的治疗方法是否能改善行为，目前尚不清楚。我们测试了通过局部注射软骨素酶ABC（ChABC）增强同侧皮质的可塑性是否能促进大鼠前肢运动皮质局灶性缺血后熟练运动功能的恢复。通过熟练抓握测试，我们发现急性和延迟ChABC治疗可诱导治疗组大鼠受损运动技能的恢复。vGLUT1、vGLUT2和vGAT染色表明，急性ChABC治疗后的功能恢复与VGLUT2阳性的兴奋性皮质回路的局部可塑性改变有关。ChABC对vGLUT2染色的影响仅出现在接受行为训练的大鼠中。因此，针对病灶周围区域细胞外基质中CSPG成分的治疗与康复相结合可能足以增强局灶性中风后的功能恢复。
