Sapalidis Konstantinos, Papavramidis Theodossis S, Gialamas Eleftherios, Deligiannidis Nikolaos, Tzioufa Valentini, Papavramidis Spiros
3rd Department of Surgery, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Emerg Trauma Shock. 2013 Jul;6(3):203-8. doi: 10.4103/0974-2700.115346.
Allopurinol acts protectively in the ischemia reperfusion injury of the small intestine. The aim of this experimental study is to define the ideal time of administration of allopurinol, in experimental models of ischemia/reperfusion.
We used 46 rabbits that were divided into four groups. Group A was the control. In Group B allopurinol was administered 10 min before ischemia and in Group C 2 min before reperfusion. In Group D, allopurinol was administered before ischemia and before reperfusion in half doses. Blood samples were collected at three different moments: (t1) prior to ischemia, (t2) prior to reperfusion, and (t3) after the end of the reperfusion, in order to determine superoxide dismutase (SOD) and neopterin values. Specimens of the intestine were obtained for histological analysis and determination of malondialdehyde (MDA).
In Group A, mucosal lesions were more extensive compared to those of the other three groups. Similarly, MDA, SOD and neopterin values were significantly higher. On the contrary, Group D showed the mildest mucosal lesions, as well as the lowest MDA, SOD and neopterin values. Finally, the lesions and the above mentioned values were bigger in Group C than in Group D.
The administration of allopurinol attenuates the production and damage effect of free oxygen radicals during ischemia reperfusion of the small intestine, thus protecting the intestinal mucosa. Its maximum beneficial action is achieved when administered both before ischemia and before reperfusion of the small intestine.
别嘌醇对小肠缺血再灌注损伤具有保护作用。本实验研究的目的是在缺血/再灌注实验模型中确定别嘌醇的理想给药时间。
我们使用了46只兔子，将其分为四组。A组为对照组。B组在缺血前10分钟给予别嘌醇，C组在再灌注前2分钟给予别嘌醇。D组在缺血前和再灌注前给予半剂量的别嘌醇。在三个不同时间点采集血样：（t1）缺血前、（t2）再灌注前、（t3）再灌注结束后，以测定超氧化物歧化酶（SOD）和新蝶呤值。获取小肠标本进行组织学分析并测定丙二醛（MDA）。
与其他三组相比，A组的黏膜损伤更广泛。同样，MDA、SOD和新蝶呤值显著更高。相反，D组的黏膜损伤最轻微，MDA、SOD和新蝶呤值也最低。最后，C组的损伤及上述指标值比D组更大。
别嘌醇给药可减轻小肠缺血再灌注期间游离氧自由基的产生和损伤作用，从而保护肠黏膜。在小肠缺血前和再灌注前均给药时可实现其最大有益作用。
