液相色谱法分离生物蛋白质。
Separation of biological proteins by liquid chromatography.
机构信息
Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi 110 025, India.
出版信息
Saudi Pharm J. 2010 Apr;18(2):59-73. doi: 10.1016/j.jsps.2010.02.001. Epub 2010 Feb 13.
After the success of human genome project, proteome is a new emerging field of biochemistry as it provides the knowledge of enzymes (proteins) interactions with different body organs and medicines administrated into human body. Therefore, the study of proteomics is very important for the development of new and effective drugs to control many lethal diseases. In proteomics study, analyses of proteome is essential and significant from the pathological point of views, i.e., in several serious diseases such as cancer, Alzheimer's disease and aging, heart diseases and also for plant biology. The separation and identification of proteomics is a challenging job due to their complex structures and closely related physico-chemical behaviors. However, the recent advances in liquid chromatography make this job easy. Various kinds of liquid chromatography, along with different detectors and optimization strategies, have been discussed in this article. Besides, attempts have been made to include chirality concept in proteomics for understanding mechanism and medication of various disease controlled by different body proteins.
人类基因组计划成功后，蛋白质组学作为一个新的生物化学领域正在兴起，因为它提供了关于酶（蛋白质）与不同身体器官和药物在人体内相互作用的知识。因此，蛋白质组学的研究对于开发新的有效药物来控制许多致命疾病非常重要。在蛋白质组学研究中，从病理学角度来看，对蛋白质组的分析是必不可少的，例如在癌症、老年痴呆症和衰老、心脏病等几种严重疾病中，以及在植物生物学中。由于其复杂的结构和密切相关的物理化学行为，蛋白质组学的分离和鉴定是一项具有挑战性的工作。然而，液相色谱技术的最新进展使得这项工作变得容易了。本文讨论了各种液相色谱技术以及不同的检测器和优化策略。此外，还尝试在蛋白质组学中引入手性概念，以了解不同蛋白质控制的各种疾病的机制和药物治疗。
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