Siddhartha Institute of Pharmacy, Dehra Dun, Uttarakhand, India.
Saudi Pharm J. 2012 Oct;20(4):365-70. doi: 10.1016/j.jsps.2012.05.012. Epub 2012 Jun 9.
Effect of Metformin in chemically induced hepatocarcinogenesis was assessed in Wistar rats. Intraperitoneal administration of chemical carcinogen diethyl nitrosamine (DENA) (200 mg/kg) in single dose elevated the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG) and reduced high density lipoproteins (HDL), total proteins (TPR) and blood glucose level in tested animals. Histopathological examinations of the liver tissue showed marked carcinogenicity of the chemical carcinogen. Food and water intake, animal weights and serum albumin (ALB) were also assessed. The animals exposed to DENA showed a significant decrease in the body weights and, there were no significant alterations found in the total bilirubin (TBR) levels and gamma-glutamyltranspeptidase (GGTP), whereas the decreased levels of serum ALB were maintained by Metformin treatment. The elevated levels of serum SGOT, SGPT, ALP, AFP, TC and TG were restored by administration of Metformin in reduced dose (125 mg/kg) daily for 16 weeks p.o. Physiological and biochemical analysis showed the beneficial effects of Metformin in the animals exposed to DENA.
二甲双胍对化学诱导肝癌发生的影响在 Wistar 大鼠中进行了评估。单次腹腔内给予化学致癌剂二乙基亚硝胺（DENA）（200mg/kg），可使血清谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）、碱性磷酸酶（ALP）、总胆固醇（TC）、甘油三酯（TG）和高密度脂蛋白（HDL）水平升高，同时降低总蛋白（TPR）和血糖水平。肝组织的组织病理学检查显示化学致癌剂具有明显的致癌性。还评估了食物和水的摄入、动物体重和血清白蛋白（ALB）。暴露于 DENA 的动物体重显著下降，总胆红素（TBR）水平和γ-谷氨酰转肽酶（GGTP）没有明显变化，而二甲双胍治疗可维持血清 ALB 水平降低。每天口服低剂量（125mg/kg）二甲双胍 16 周可使血清 SGOT、SGPT、ALP、AFP、TC 和 TG 水平升高得到恢复。生理生化分析显示，二甲双胍对暴露于 DENA 的动物具有有益作用。
