Siddhartha Institute of Pharmacy, Dobachi, Near IT Park, Dehra Dun, 248001, Uttarakhand, India.
Mol Cell Biochem. 2013 Dec;384(1-2):279-85. doi: 10.1007/s11010-013-1811-7. Epub 2013 Sep 20.
Sulfonamides have been reported to possess substantial antitumor activity as they act as carbonic anhydrase inhibitors. In addition, selenium appears to have a protective effect at various stages of cancer due to its antioxidant property, enhanced carcinogen detoxification, inhibition of cell invasion, and by inhibiting angiogenesis. Here, in the present study we aimed to evaluate and synergize the cytotoxic activity of sulfonamide and selenium (SM+SE) as effective therapy in the treatment of DENA-induced HCC. Hepatocarcinogeneis was induced by a single intraperitoneal injection of diethylnitrosamine (DENA) (200 mg/kg) in phosphate buffer. 30 Male Wistar rats used in this study were divided randomly into five equal groups (n = 6). DENA-administered animals showed significant alteration (p < 0.001) in liver-specific enzymes-glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), and Alpha fetoproteins (AFP), and also induced severe histopathological changes in the hepatic tissues. Interestingly, treatment with (SE+SE) (SM 30 mg/kg + SE 3 mg/kg) significantly reduced (P < 0.001, P < 0.001, P < 0.001, P < 0.001) the elevated AFP, SGOT, SGPT, and ALP levels, respectively, suggesting that combination therapy of SM+SE has a potential to treat DENA-induced liver damage.
磺胺类药物被报道具有显著的抗肿瘤活性，因为它们是碳酸酐酶抑制剂。此外，由于其抗氧化特性、增强的致癌物解毒、抑制细胞侵袭和抑制血管生成，硒似乎在癌症的各个阶段都具有保护作用。在这里，在本研究中，我们旨在评估磺胺和硒（SM+SE）的细胞毒性活性并将其协同作用作为治疗 DENA 诱导的 HCC 的有效疗法。通过在磷酸盐缓冲液中单次腹腔注射二乙基亚硝胺（DENA）（200mg/kg）诱导肝癌发生。本研究使用的 30 只雄性 Wistar 大鼠随机分为五组（n=6）。DENA 给药动物的肝特异性酶-谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）、碱性磷酸酶（ALP）和甲胎蛋白（AFP）显著改变（p<0.001），并且肝组织也发生了严重的组织病理学变化。有趣的是，用（SE+SE）（SM 30mg/kg+SE 3mg/kg）治疗可显著降低（P<0.001、P<0.001、P<0.001、P<0.001）分别升高的 AFP、SGOT、SGPT 和 ALP 水平，表明 SM+SE 的联合治疗具有治疗 DENA 诱导的肝损伤的潜力。
