Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdul-Aziz University-Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2011 Jan;18(1):107-11. doi: 10.1016/j.sjbs.2010.10.006. Epub 2010 Oct 28.
There is strong evidence that supports the role of tumour necrosis factors (TNF-alpha/beta) as common genetic factors, located on 6p21.1-6p21.3 loci, in the pathogenesis of asthma disease. In this study, we extended our research work on TNFA to include the genotyping of Saudi asthmatic children as regards to TNFB gene (namely as lymphotoxin-α, LTA). We examined 60 asthmatic Saudi children compared to 56 healthy non-asthmatics using the PCR-RFLP analyses to identify the polymorphism +252A>G in intron 1 in lymphotoxin-α gene. We identified 55% of the allele A of the LTA∗NcoI polymorphism in subjects with asthma disease, and 45% of the allele G. In this study, the frequency of the LTA∗NcoI-A/A genotype was 40% preferably to the LTA∗NcoI-G/A and LTA∗NcoI-G/G genotypes. In addition, the severe persistent asthmatic cases were associated with the LTA∗NcoI-AA genotype at a frequency of 80%, while the genotype LTA∗NcoI-GG are associated with the mildest form of the disease. Consequently, one could predict the severity of asthma and hence the polymorphism of the LTA∗NcoI. Herein, we stated that more than 93% of Saudi children under investigation lived in the randomized areas of western regions of Saudi Arabia. In conclusion, genotype frequencies for the LTA+252 polymorphisms were significantly different from the controls. These findings may have implications for future early intervention studies by helping to identify infants at increased risk for wheezing and childhood asthma.
有强有力的证据表明，肿瘤坏死因子（TNF-α/β）作为共同的遗传因素，位于 6p21.1-6p21.3 基因座，在哮喘病的发病机制中起作用。在这项研究中，我们将我们对 TNFA 的研究工作扩展到包括对沙特哮喘儿童的 TNFB 基因（即淋巴毒素-α，LTA）进行基因分型。我们使用 PCR-RFLP 分析方法检查了 60 名沙特哮喘儿童和 56 名健康非哮喘者，以确定淋巴毒素-α基因内含子 1 中的 +252A>G 多态性。我们在哮喘病患者中鉴定出 LTA∗NcoI 多态性的等位基因 A 的 55%，等位基因 G 的 45%。在这项研究中，LTA∗NcoI-A/A 基因型的频率优选为 40%，而 LTA∗NcoI-G/A 和 LTA∗NcoI-G/G 基因型的频率为 60%。此外，严重持续性哮喘病例与 LTA∗NcoI-AA 基因型相关，其频率为 80%，而 LTA∗NcoI-GG 基因型与疾病的最轻微形式相关。因此，可以预测哮喘的严重程度，从而预测 LTA∗NcoI 的多态性。在此，我们指出，在所调查的 93%以上的沙特儿童生活在沙特阿拉伯西部地区的随机地区。总之，LTA+252 多态性的基因型频率与对照组有显著差异。这些发现可能对未来的早期干预研究具有重要意义，有助于识别易患喘息和儿童哮喘的婴儿。
