Department of Biotechnology, Cochin University of Science and Technology, Cochin 682022, Kerala, India.
Saudi J Biol Sci. 2013 Apr;20(2):205-12. doi: 10.1016/j.sjbs.2013.02.002. Epub 2013 Feb 20.
Halobacteria, members of the domain Archaea that live under extremely halophilic conditions, are often considered as dependable source for deriving novel enzymes, novel genes, bioactive compounds and other industrially important molecules. Protein antibiotics have potential for application as preserving agents in food industry, leather industry and in control of infectious bacteria. Halocins are proteinaceous antibiotics synthesized and released into the environment by extreme halophiles, a universal characteristic of halophilic bacteria. Herein, we report the production of halocin (SH10) by an extremely halophilic archeon Natrinema sp. BTSH10 isolated from salt pan of Kanyakumari, Tamilnadu, India and optimization of medium for enhanced production of halocin. It was found that the optimal conditions for maximal halocin production were 42 °C, pH 8.0, and 104 h of incubation at 200 rpm with 2% (V/V) inoculum concentration in Zobell's medium containing 3 M NaCl, Galactose, beef extract, and calcium chloride as additional supplements. Results indicated scope for fermentation production of halocin for probable applications using halophilic archeon Natrinema sp. BTSH10.
嗜盐菌是生活在极端嗜盐环境中的古菌域成员，通常被认为是获取新型酶、新型基因、生物活性化合物和其他工业上重要分子的可靠来源。蛋白抗生素具有作为食品工业、制革工业中的防腐剂以及控制感染性细菌的应用潜力。卤菌素是由极端嗜盐菌合成并释放到环境中的蛋白抗生素，是嗜盐细菌的普遍特征。在此，我们报道了一种从印度泰米尔纳德邦坎亚库马里盐田分离的极端嗜盐古菌 Natrinema sp. BTSH10 生产卤菌素 (SH10)，并优化了培养基以提高卤菌素的产量。结果发现，在含有 3 M NaCl、半乳糖、牛肉提取物和氯化钙的 Zobell 培养基中，最佳产卤菌素条件为 42°C、pH8.0、200 rpm 孵育 104 h，接种量为 2%（V/V）。结果表明，使用嗜盐古菌 Natrinema sp. BTSH10 进行卤菌素发酵生产具有可能的应用前景。
