Taneja Isha, Erukala Madhuri, Raju Kanumuri Siva Rama, Singh Sheelendra Pratap
Academy of Scientific & Industrial Research, New Delhi, India.
Bioanalysis. 2013 Sep;5(17):2171-86. doi: 10.4155/bio.13.180.
Malaria is the leading parasitic disease in emerging countries. Therapeutic drug monitoring of antimalarial drugs is becoming increasingly important due to their spreading resistance. Measuring systemic antimalarial drug concentrations is also vital for safety and PK evaluations during clinical development. The dried blood spot (DBS) technique is a convenient alternative sample-collection method to venipuncture, especially in resource -limited areas where the clinical studies of antimalarials are usually carried out. Various bioanalytical methods for antimalarial drug estimation utilizing DBS sampling have been reported. This review discusses the applicability and relevance of DBS in quantitative assessment of antimalarial drugs, the advantages and drawbacks of DBS, and the difficulties encountered during its implementation.
疟疾是新兴国家中主要的寄生虫病。由于抗疟药物耐药性的不断蔓延，对抗疟药物进行治疗药物监测变得越来越重要。在临床开发过程中，测量全身抗疟药物浓度对于安全性和药代动力学评估也至关重要。干血斑（DBS）技术是一种方便的替代静脉穿刺的样本采集方法，特别是在通常开展抗疟药物临床研究的资源有限地区。已经报道了多种利用DBS采样进行抗疟药物估算的生物分析方法。本文综述讨论了DBS在抗疟药物定量评估中的适用性和相关性、DBS的优缺点以及实施过程中遇到的困难。
