Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:17-55. doi: 10.1016/j.pnmrs.2013.07.001. Epub 2013 Jul 20.
A general review of novel NMR methods to measure heteronuclear long-range proton-carbon coupling constants ((n)JCH; n>1) in small molecules is made. NMR experiments are classified in terms of NMR pulse scheme and cross-peak nature. A discussion about simplicity, general applicability and accuracy for each particular NMR experiment is presented and exemplified. Important aspects such as the sign determination and measurement of very small coupling values involving protonated and non-protonated carbons as well as the complementarity between different experiments are also discussed. Finally, a compilation of applications in structural and conformational analysis of different types of molecules since 2000 is surveyed.
本文综述了测定小分子中异核远程质子-碳耦合常数（(n)JCH；n>1）的新型 NMR 方法。根据 NMR 脉冲方案和交叉峰性质对 NMR 实验进行分类。本文介绍并举例说明了每种特定 NMR 实验的简单性、通用性和准确性。还讨论了一些重要方面，如涉及质子化和非质子化碳的非常小的耦合值的符号确定和测量，以及不同实验之间的互补性。最后，调查了 2000 年以来不同类型分子的结构和构象分析中的应用情况。
