Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain.
Org Biomol Chem. 2013 Jul 21;11(27):4473-8. doi: 10.1039/c3ob40675j.
A user-friendly 2D NMR approach denoted as CLIP-HSQMBC is proposed for the very easy, direct and accurate measurement of long-range proton-carbon coupling constants in organic molecules and natural products. The J value can be extracted directly from the analysis of resolved in-phase (1)H multiplets that show an additional splitting arising from the proton-carbon coupling. In cases of unresolved peaks, a simple fitting analysis using the internal satellite lines as a reference is performed. Addition of a spin-lock period results in a CLIP-HSQMBC-TOCSY experiment that is suitable for the measurement of very small coupling values or to observe correlations from overlapped resonances.
提出了一种易于使用的二维 NMR 方法 CLIP-HSQMBC，用于非常简单、直接和准确地测量有机分子和天然产物中的长程质子-碳耦合常数。可以直接从分析显示出由于质子-碳耦合而产生的附加分裂的分辨同相（1）H 多重峰中提取 J 值。在未分辨峰的情况下，使用内部卫星线作为参考进行简单的拟合分析。添加自旋锁定周期会得到 CLIP-HSQMBC-TOCSY 实验，该实验适用于测量非常小的耦合值或观察来自重叠共振的相关。
