采用高效液相色谱法测定全血、血浆和尿液中的喷他脒。

Determination of pentamidine in whole blood, plasma, and urine by high-performance liquid chromatography.

作者信息

Ericsson O, Rais M

机构信息

Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.

出版信息

Ther Drug Monit. 1990 Jul;12(4):362-5. doi: 10.1097/00007691-199007000-00011.

DOI:10.1097/00007691-199007000-00011

PMID:2396310

Abstract

The analysis of pentamidine in whole blood, plasma, and urine by liquid chromatography is described. Extraction was made with a mixture of acetonitrile and chloroform followed by back-extraction into phosphate buffer. A reversed-phase chromatographic system with fluorescence detection was used. The precision of the method was 5-7% at the lower limit of determination (16 nmol/L in plasma and hemolyzed whole blood, 27.7 nmol/L in urine).

摘要

描述了通过液相色谱法分析全血、血浆和尿液中的喷他脒。用乙腈和氯仿的混合物进行萃取，然后反萃取到磷酸盐缓冲液中。使用了带有荧光检测的反相色谱系统。该方法在测定下限（血浆和溶血全血中为16 nmol/L，尿液中为27.7 nmol/L）时的精密度为5 - 7%。