Berger B J, Hall J E, Tidwell R R
Department of Parasitology, School of Public Health, University of North Carolina, Chapel Hill 27599.
J Chromatogr. 1989 Sep 29;494:191-200. doi: 10.1016/s0378-4347(00)82668-7.
A high-performance liquid chromatographic method has been developed for the detection and quantification of pentamidine and pentamidine analogues of chemotherapeutic value in order to measure their concentration in physiological fluids. The compounds were extracted from urine over octadecyl solid-phase extraction columns, followed by chromatographic separation with an octadecyl reversed-phase column. For the mobile phase, a gradient of 31.5-37.5% acetonitrile in water, with sodium heptanesulfonate and tetramethylammonium chloride as ion modifiers, was used. This method was used to reliably detect levels as low as 341 ng/ml without concentration of the compounds during the solid-phase extraction. The assay was used to determine the effectiveness of several solid-phase extraction columns for isolating the compounds of interest and to quantify the amount of pentamidine and its analogues contained in the urine of dosed rats.
已开发出一种高效液相色谱法，用于检测和定量具有化疗价值的喷他脒及其类似物，以便测量它们在生理体液中的浓度。这些化合物通过十八烷基固相萃取柱从尿液中萃取出来，然后用十八烷基反相柱进行色谱分离。对于流动相，使用了水相中31.5 - 37.5%乙腈的梯度，其中庚烷磺酸钠和四甲基氯化铵作为离子改性剂。该方法用于可靠地检测低至341 ng/ml的水平，而在固相萃取过程中无需对化合物进行浓缩。该分析方法用于确定几种固相萃取柱分离目标化合物的有效性，并定量给药大鼠尿液中所含喷他脒及其类似物的量。
