通过小分子连接的 DNA 的末端保护和 Exo III 辅助的 DNA 循环扩增实现小分子-蛋白质相互作用的超灵敏检测。
Ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification.
机构信息
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
出版信息
Chem Commun (Camb). 2013 Oct 9;49(78):8854-6. doi: 10.1039/c3cc43621g.
PMID:23964359
Abstract
Terminal protection of small molecule linked DNA assays amplified by Exo III-aided DNA recycling is developed for the detection of streptavidin-biotin interaction, with a detection limit of 0.8 fM streptavidin in the "turn-on" state, indicating that the proposed method is an ultrasensitive platform for the detection of small molecule-protein interactions.
摘要
开发了一种基于 Exo III 辅助 DNA 循环的小分子连接 DNA 检测的末端保护方法，用于检测链霉亲和素-生物素相互作用，在“开启”状态下对链霉亲和素有 0.8 fM 的检测限，表明该方法是一种用于检测小分子-蛋白质相互作用的超灵敏平台。
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