一种基于核酸外切酶III辅助靶标循环扩增的无标记且开启式荧光策略用于宽检测范围的DNA检测。
A Label-free and Turn-on Fluorescence Strategy for DNA Detection with a Wide Detection Range Based on Exonuclease III-aided Target Recycling Amplification.
作者信息
Yang Hualin, Song Xiaoda, Ding Baomiao, Li Zhenshun, Zhang Xingping
机构信息
Edible and Medicinal Fungi Research Center, College of Life Science, Yangtze University.
出版信息
Anal Sci. 2017;33(1):9-11. doi: 10.2116/analsci.33.9.
In the present work, a simple and sensitive turn-on fluorescence method for DNA detection was developed. It was explored based on the N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter and exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. Our method showed an ultra-wide detection range from 10 fM to 100 nM with a low linear detection limit of 0.76 nM. It also had excellent selectivity in a selectivity experiment.
在本研究中，开发了一种用于DNA检测的简单且灵敏的荧光开启方法。该方法基于N-甲基中卟啉IX（NMM）/G-四链体DNA系统作为报告物，并借助核酸外切酶III（Exo III）辅助的靶标循环扩增来确保灵敏度。我们的方法显示出从10 fM到100 nM的超宽检测范围，线性检测下限低至0.76 nM。在选择性实验中它也具有出色的选择性。
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