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Expert Opin Drug Discov. 2013 Oct;8(10):1191-202. doi: 10.1517/17460441.2013.825244. Epub 2013 Aug 21.
Apoptosis has become an important target for drug discovery. Although the mouse remains the animal model of choice for the preclinical assessment of drug toxicity and efficacy, zebrafish are increasingly used for early drug studies. Here, we describe approaches for assessing drug effects on apoptosis in transparent zebrafish.
In this review, the authors discuss the drug effects on developmentally regulated apoptosis using microscopy. They also discuss the effects of neuroprotectants in a chemical induced disease model using morphometric image analysis. Finally, the authors review the effects of radioprotectants in irradiated whole animals using a conventional 96-well microplate format.
Several challenges have limited more widespread use of zebrafish as the organism of choice for drug screening. However, zebrafish do have a number of compelling inherent advantages including: rapid organogenesis, transparency, statistically significant numbers of animals per experiment and adaptability of cell-based methods.
细胞凋亡已成为药物研发的重要靶点。尽管小鼠仍然是药物毒性和疗效临床前评估的首选动物模型，但斑马鱼越来越多地用于早期药物研究。在这里，我们描述了评估药物对透明斑马鱼细胞凋亡影响的方法。
在这篇综述中，作者讨论了使用显微镜评估药物对发育调控细胞凋亡的影响。他们还讨论了形态计量图像分析在化学诱导疾病模型中神经保护剂的作用。最后，作者综述了辐射保护剂在辐照全动物中的作用，使用传统的 96 孔微孔板格式。
有几个挑战限制了斑马鱼更广泛地被用作药物筛选的首选生物。然而，斑马鱼确实具有许多引人注目的固有优势，包括：快速器官发生、透明性、实验中具有统计学意义的大量动物数量以及基于细胞方法的适应性。
