Delvecchio Chris, Tiefenbach Jens, Krause Henry M
Banting and Best Department of Medical Research, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Canada.
Assay Drug Dev Technol. 2011 Aug;9(4):354-61. doi: 10.1089/adt.2010.0346. Epub 2011 Feb 10.
The zebrafish (Danio rerio) is an emerging vertebrate model for drug discovery that permits whole animal drug screens with excellent throughput, combined with ease of use and low cost. This review will begin with a discussion on the background, suitability, and advantages of this vertebrate model system and then, citing specific examples, will describe the utility of zebrafish at specific stages in the drug development pipeline. We will end with a synopsis of recent drug screens based on morphological disruptions, genetic disease models, fluorescent markers, behavioral changes, and specific targets. The numerous advantages of this whole animal approach provide new promise for the discovery of safe, specific, and powerful new drugs.
斑马鱼（Danio rerio）是一种新兴的用于药物发现的脊椎动物模型，它能够进行全动物药物筛选，通量极高，且易于使用、成本低廉。本综述将首先讨论这个脊椎动物模型系统的背景、适用性和优势，然后引用具体实例，描述斑马鱼在药物研发流程特定阶段的应用。我们将以基于形态学破坏、遗传疾病模型、荧光标记、行为变化和特定靶点的近期药物筛选综述作为结尾。这种全动物方法的众多优势为发现安全、特效且强效的新药带来了新的希望。
