Scholtens Ingrid, Laurensse Emile, Molenaar Bonnie, Zaaijer Stephanie, Gaballo Heidi, Boleij Peter, Bak Arno, Kok Esther
RIKILT Wageningen University and Research Centre, P.O. Box 230, 6700 AE Wageningen, The Netherlands.
J Agric Food Chem. 2013 Sep 25;61(38):9097-109. doi: 10.1021/jf4018146. Epub 2013 Sep 10.
Nowadays most animal feed products imported into Europe have a GMO (genetically modified organism) label. This means that they contain European Union (EU)-authorized GMOs. For enforcement of these labeling requirements, it is necessary, with the rising number of EU-authorized GMOs, to perform an increasing number of analyses. In addition to this, it is necessary to test products for the potential presence of EU-unauthorized GMOs. Analysis for EU-authorized and -unauthorized GMOs in animal feed has thus become laborious and expensive. Initial screening steps may reduce the number of GMO identification methods that need to be applied, but with the increasing diversity also screening with GMO elements has become more complex. For the present study, the application of an informative detailed 24-element screening and subsequent identification strategy was applied in 50 animal feed samples. Almost all feed samples were labeled as containing GMO-derived materials. The main goal of the study was therefore to investigate if a detailed screening strategy would reduce the number of subsequent identification analyses. An additional goal was to test the samples in this way for the potential presence of EU-unauthorized GMOs. Finally, to test the robustness of the approach, eight of the samples were tested in a concise interlaboratory study. No significant differences were found between the results of the two laboratories.
如今，大多数进口到欧洲的动物饲料产品都带有转基因生物（GMO）标签。这意味着它们含有欧盟（EU）授权的转基因生物。为了执行这些标签要求，随着欧盟授权的转基因生物数量不断增加，需要进行越来越多的分析。除此之外，还需要检测产品中是否可能存在未经欧盟授权的转基因生物。因此，对动物饲料中欧盟授权和未经授权的转基因生物进行分析变得既费力又昂贵。初步筛选步骤可能会减少需要应用的转基因生物鉴定方法的数量，但随着多样性的增加，使用转基因生物元件进行筛选也变得更加复杂。在本研究中，对50个动物饲料样本应用了一种信息丰富的详细24元件筛选及后续鉴定策略。几乎所有饲料样本都被标记为含有转基因来源的材料。因此，该研究的主要目的是调查详细的筛选策略是否会减少后续鉴定分析的数量。另一个目的是以这种方式检测样本中是否可能存在未经欧盟授权的转基因生物。最后，为了测试该方法的稳健性，在一项简洁的实验室间研究中对其中8个样本进行了测试。两个实验室的结果之间未发现显著差异。
