Department of Medical Research, Genetics Center, China Medical University Hospital , Taichung , Taiwan .
Ren Fail. 2013;35(10):1370-5. doi: 10.3109/0886022X.2013.828264. Epub 2013 Aug 21.
The purpose of this study was to determine whether toll-like receptors 9 (TLR9) gene polymorphisms (rs352139 and rs352140) were markers of susceptibility to the development and progression of membranous nephropathy (MGN) in Taiwanese patients. The polymorphisms were investigated by polymerase chain reaction in 397 Taiwanese individuals (134 MGN patients and 263 controls). Patients with malignancy, chronic infectious diseases, lupus nephritis, or drug-induced secondary MGN were excluded from the study. Data showed AA genotype at rs352139 SNP or GG genotype at rs352140 SNP may indicate higher risk for MGN (odds ratio [OR] = 1.55; 95% confidence interval [CI] = 1.02-2.35, at rs352139 SNP; OR = 1.57; 95% CI = 1.03-2.39, at rs352140 SNP). However, MGN patients with A-G haplotype were susceptible for decreased creatinine clearance rate and for seriously tubule-interstitial fibrosis. The result suggests for the first time that TLR9 (rs352139 and rs352140) polymorphisms may contribute to the development and progression of MGN.
本研究旨在确定 Toll 样受体 9（TLR9）基因多态性（rs352139 和 rs352140）是否可作为台湾患者发生和进展为膜性肾病（MGN）的易感性标志物。通过聚合酶链反应对 397 名台湾个体（134 名 MGN 患者和 263 名对照）进行了多态性研究。本研究排除了患有恶性肿瘤、慢性传染病、狼疮性肾炎或药物诱导的继发性 MGN 的患者。数据显示，rs352139 SNP 的 AA 基因型或 rs352140 SNP 的 GG 基因型可能表明 MGN 的风险更高（比值比 [OR] = 1.55；95%置信区间 [CI] = 1.02-2.35，rs352139 SNP；OR = 1.57；95% CI = 1.03-2.39，rs352140 SNP）。然而，具有 A-G 单倍型的 MGN 患者易发生肌酐清除率降低和严重肾小管间质纤维化。该结果首次表明 TLR9（rs352139 和 rs352140）多态性可能有助于 MGN 的发生和进展。
