Ag+介导的 DNA 碱基配对：非常稳定的吡咯-dC-吡咯-dC 碱基对结合两个银离子。
Ag+-mediated DNA base pairing: extraordinarily stable pyrrolo-dC-pyrrolo-dC pairs binding two silver ions.
机构信息
Laboratory of Bioorganic Chemistry and Chemical Biology, Center for Nanotechnology , Heisenbergstraße 11, 48149 Münster, Germany.
出版信息
J Org Chem. 2013 Sep 20;78(18):9457-63. doi: 10.1021/jo401109w. Epub 2013 Sep 10.
6-Substituted pyrrolo-dC-pyrrolo-dC mismatches selectively capture silver ions to form extraordinarily stable metal-mediated base pairs. One single modification in a 12-mer duplex causes a Tm increase of 36.0 °C relative to the metal-free mismatched duplex. Spectrophotometric titrations as well as ESI mass spectra confirmed the binding of two silver ions per base pair. The Ag(+)-mediated base pairs may permit the construction of metal-responsive DNA with a very high silver loading.
6-取代的吡咯并-dC-吡咯并-dC 错配物选择性地捕获银离子，形成非常稳定的金属介导碱基对。在 12 个碱基对的双链体中仅进行一次修饰，就会使 Tm 值相对于无金属的错配双链体增加 36.0°C。分光光度滴定和 ESI 质谱证实每个碱基对结合了两个银离子。Ag(+)介导的碱基对可能允许构建具有非常高银负载的金属响应性 DNA。
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