Department of General Surgery, E-Da Hospital, I-Shou University, Kaohsiung 82445, Taiwan.
Int J Mol Sci. 2013 Aug 20;14(8):17147-56. doi: 10.3390/ijms140817147.
Human oral squamous cell carcinoma (OSCC) is a common form of malignant cancer, for which radiotherapy or chemotherapy are the main treatment methods. Cucurbitacin E (CuE) is a natural compound previously shown to be an antifeedant as well as a potent chemopreventive agent against several types of cancer. The present study investigates anti-proliferation (using MTT assay, CuE demonstrated cytotoxic activity against SAS cell with IC50 values at 3.69 µM) and induced apoptosis of human oral squamous cell carcinoma SAS cells after 24 h treatment with CuE. Mitochondrial membrane potential (MMP) and caspase activity were studied and our results indicate that CuE inhibits cell proliferation as well as the activation of apoptois in SAS cells. Both effects increased in proportion to the dosage of CuE and apoptosis was induced via mitochondria- and caspase-dependent pathways. CuE can induce cell death by a mechanism that is not dependent on apoptosis induction, and thus represents a promising anticancer agent for prevention and treatment of OSCC.
人口腔鳞状细胞癌（OSCC）是一种常见的恶性肿瘤，其主要治疗方法为放疗或化疗。葫芦素 E（CuE）是一种天然化合物，先前已被证明具有抗营养作用，也是多种癌症的有效化学预防剂。本研究探讨了 CuE 对人口腔鳞状细胞癌 SAS 细胞的抗增殖作用（MTT 检测结果表明，CuE 对 SAS 细胞具有细胞毒性，IC50 值为 3.69 μM）和诱导凋亡作用。研究了线粒体膜电位（MMP）和半胱氨酸天冬氨酸蛋白酶（caspase）活性，结果表明 CuE 抑制 SAS 细胞的增殖和凋亡激活。这两种作用均随 CuE 剂量的增加而增加，凋亡是通过线粒体和半胱氨酸天冬氨酸蛋白酶依赖性途径诱导的。CuE 可以通过不依赖凋亡诱导的机制诱导细胞死亡，因此代表了预防和治疗 OSCC 的一种有前途的抗癌药物。
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