Dept. of Bioengineering, University of California, San Diego, La Jolla, California.
Biotechnol Prog. 2013 Nov-Dec;29(6):1565-72. doi: 10.1002/btpr.1797. Epub 2013 Sep 10.
Perfluorocarbon (PFC) emulsions can transport and release various gases based on concentration gradients. The objective of this study was to determine the possibility of carrying and delivering exogenous nitric oxide (NO) into the circulation by simply loading PFC emulsion with NO prior infusion. PFC was equilibrated with room air (PFC) or 300 ppm NO (PFC-NO) at atmospheric pressure. Isotonic saline solution was used as a volume control (Saline). PFC and PFC-NO were infused at a dose of 3.5 mL/kg in the hamster window chamber model. Blood chemistry, and systemic and microvascular hemodynamic response were measured. Infusion of PFC preloaded with NO reduced blood pressure, induced microvascular vasodilation and increased capillary perfusion; although these changes lasted less than 30 min post infusion. On the other hand, infusion of PFC (without NO) produced vasoconstriction; however, the vasoconstriction was followed by vasodilatation at 30 min post infusion. Plasma nitrite and nitrate increased 15 min after infusion of NO preloaded PFC compared with PFC, 60 min after infusion nitrite and nitrate were not different, and 90 min after infusion plasma S-nitrosothiols increased in both groups. Infusion of NO preloaded PFC resulted in acute vascular relaxation, where as infusion of PFC (without NO) produced vasoconstriction, potentially due to NO sequestration by the PFC micelles. The late effects of PFC infusion are due to NO redistribution and plasma S-nitrosothiols. Gas solubility in PFC can provide a tool to modulate plasma vasoactive NO forms availability and improve microcirculatory function and promote increased blood flow.
全氟碳（PFC）乳液可以根据浓度梯度输送和释放各种气体。本研究的目的是通过在输注前将 PFC 乳液简单地加载外源一氧化氮（NO）来确定将外源性 NO 携带并递送到循环中的可能性。PFC 在大气压下与室内空气（PFC）或 300 ppm NO（PFC-NO）平衡。等渗盐水用作容量控制（盐水）。在仓鼠窗室模型中以 3.5 mL/kg 的剂量输注 PFC 和 PFC-NO。测量血液化学、全身和微血管血液动力学反应。预加载 NO 的 PFC 输注降低血压，诱导微血管扩张并增加毛细血管灌注；尽管这些变化在输注后不到 30 分钟持续。另一方面，输注不含 NO 的 PFC 会引起血管收缩；然而，在输注后 30 分钟，血管收缩后会出现血管扩张。与 PFC 相比，NO 预加载 PFC 输注后 15 分钟血浆亚硝酸盐和硝酸盐增加，输注后 60 分钟亚硝酸盐和硝酸盐无差异，输注后 90 分钟两组血浆 S-亚硝基硫醇均增加。预加载 NO 的 PFC 输注导致急性血管松弛，而 PFC（不含 NO）输注导致血管收缩，这可能是由于 PFC 胶束对 NO 的隔离。PFC 输注的后期影响归因于 NO 的再分布和血浆 S-亚硝基硫醇。PFC 中的气体溶解度可以提供一种工具来调节血浆血管活性 NO 形式的可用性，改善微循环功能并促进血流量增加。
