Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama 35233, USA.
Macromol Biosci. 2013 Oct;13(10):1404-12. doi: 10.1002/mabi.201300155. Epub 2013 Aug 21.
Understanding the role of the pancreatic extracellular matrix (ECM) in supporting islet survival and function drives the pursuit to create biomaterials that imitate and restore the pancreatic ECM microenvironment. To create an ECM mimic holding bioinductive cues for β-cells, self-assembled peptide amphiphiles (PAs) inscribed with four selected ECM-derived cell adhesive ligands are synthesized. After 7 days, compared to control groups cultured on biologically inert substrates, MIN6 β-cells cultured on PAs functionalized with YIGSR and RGDS cell adhesive ligands exhibit elevated insulin secretion in responses to glucose and also form β-cell clusters. These findings suggest that the self-assembled PA nanomatrix may be utilized to improve pancreatic islet transplantation for treating type 1 diabetes.
了解胰腺细胞外基质 (ECM) 在支持胰岛存活和功能中的作用，推动了创造仿生材料的研究，以模仿和恢复胰腺 ECM 微环境。为了创建一种具有β细胞生物诱导信号的 ECM 模拟物，合成了带有四个选定的 ECM 衍生细胞黏附配体的自组装肽两亲物 (PAs)。与在生物惰性基底上培养的对照组相比，在 YIGSR 和 RGDS 细胞黏附配体功能化的 PAs 上培养的 MIN6 β 细胞在响应葡萄糖时表现出更高的胰岛素分泌，并且还形成了β细胞簇。这些发现表明，自组装 PA 纳米基质可用于改善胰岛移植治疗 1 型糖尿病。
