Biomet Biologics, 56 East Bell Drive, Warsaw, 46581, Indiana.
J Orthop Res. 2013 Dec;31(12):1929-35. doi: 10.1002/jor.22464. Epub 2013 Aug 21.
Osteoarthritis (OA) is characterized by deterioration of articular cartilage driven by an imbalance of pro- and anti-inflammatory cytokines. To address the cartilage deterioration observed in OA, an autologous protein solution (APS) has been developed which has been shown to inhibit the production of destructive proteases and inflammatory cytokines from chondrocytes and monocytes, respectively. The purpose of this study was to determine the chondroprotective effect of APS on IL-1α- or TNFα-challenged bovine articular cartilage explants. Cartilage explants were cultured in the presence or absence of recombinant inflammatory cytokines, IL-1α and TNFα. Explants under equivalent inflammatory conditions were pretreated with recombinant antagonists IL-1ra, sTNF-RI, or APS to measure their inhibition of matrix degradation. Explants were further evaluated with Safranin-O, Masson's Trichrome, and Hematoxylin and Eosin histological staining. APS was more effective than recombinant antagonists in preventing cartilage matrix degradation and inhibited any measurable IL-1α-induced collagen release over a 21-day culture period. APS treatment reduced the degree of Safranin-O staining loss when cartilage explants were cultured with IL-1α or TNFα. Micrographs of APS treated cartilage explants showed an increase in observed cellularity and apparent cell division. APS may have the potential to prevent cartilage loss associated with early OA.
骨关节炎（OA）的特征是关节软骨退化，由促炎和抗炎细胞因子失衡驱动。为了解决 OA 中观察到的软骨退化问题，已经开发了一种自体蛋白溶液（APS），它已被证明分别抑制软骨细胞和单核细胞产生破坏性蛋白酶和炎性细胞因子。本研究的目的是确定 APS 对 IL-1α 或 TNFα 刺激的牛关节软骨外植体的软骨保护作用。在存在或不存在重组炎性细胞因子 IL-1α 和 TNFα 的情况下培养软骨外植体。在等效炎性条件下的外植体用重组拮抗剂 IL-1ra、sTNF-RI 或 APS 预处理，以测量其对基质降解的抑制作用。进一步用番红 O、马松三色和苏木精和伊红组织染色评估外植体。APS 比重组拮抗剂更有效地防止软骨基质降解，并在 21 天的培养期间抑制任何可测量的 IL-1α 诱导的胶原释放。APS 处理降低了软骨外植体与 IL-1α 或 TNFα 共培养时番红 O 染色丢失的程度。APS 处理的软骨外植体的显微照片显示出细胞数量增加和明显的细胞分裂。APS 可能具有预防与早期 OA 相关的软骨损失的潜力。
