Institute of Chemical Technology, Department of Analytical Chemistry, Prague, Czech Republic.
Chirality. 2013 Nov;25(11):810-3. doi: 10.1002/chir.22220. Epub 2013 Aug 22.
Racemic mixtures of six Tröger's base derivatives were separated by chiral nonaqueous capillary electrophoresis. The separation protocol was optimized first for suitable solvents. Then the applicability of various salts dissolved in organic solvents and their mixtures was evaluated. As chiral selectors β-cyclodextrin and heptakis(2,6-di-O-methyl)-β-cyclodextrin at various concentrations were used. The best enantioselectivity for the studied analytes was obtained utilizing formamide as organic nonaqueous solvent containing a mixture of sodium citrate and tris(hydroxymethyl)aminomethane acetate as electrolytes, and β-cyclodextrin as chiral additive. The experimental results demonstrated the feasibility of nonaqueous capillary electrophoresis for enantioseparation of Tröger's base derivatives. This technique represents a suitable alternative to more commonly used capillary electrophoresis in aqueous environment.
通过手性非水毛细管电泳对六个 Tröger's 碱衍生物的外消旋混合物进行了分离。首先优化了分离方案以选择合适的溶剂。然后评估了各种盐在有机溶剂及其混合物中的应用。使用了β-环糊精和七（2,6-二-O-甲基）-β-环糊精作为手性选择剂，浓度不同。在外消旋混合物中，使用包含柠檬酸三钠和三羟甲基氨基甲烷乙酸盐的混合物的甲酰胺作为有机溶剂，以及β-环糊精作为手性添加剂，为研究的分析物获得了最佳的对映选择性。实验结果表明，非水毛细管电泳对手性 Tröger's 碱衍生物的分离具有可行性。该技术代表了在水相环境中更常用的毛细管电泳的合适替代方法。
