Institute of Chemical Technology, Department of Analytical Chemistry, Technická 5, 166 28, Prague 6, Czech Republic.
Chirality. 2013 Jul;25(7):379-83. doi: 10.1002/chir.22148. Epub 2013 Jun 6.
The enantioseparation of seven Tröger's base derivatives (TBs) was carried out by capillary electrophoresis using α-, β-, and γ-cyclodextrins as chiral selectors and phosphate at 20 mmol/l concentration, pH 2.5, as background electrolyte. The method was optimized with respect to the concentration of chosen chiral selectors (0-50 mmol/l) and the amount of organic solvent (acetonitrile, 0-25 % (v/v)) in the electrolyte. The results indicate that all the studied variables, i.e., type of chiral selector, its concentration, and the amount of the added organic solvent, have a significant impact on the enantioseparation of the studied TBs. The best results for the majority of the separated TBs were obtained utilizing β-cyclodextrin at 5 mmol/l concentration and with various amounts of acetonitrile added ranging from 5 to 15% (v/v) in the background electrolyte. For the two smallest studied TBs, γ-cyclodextrin with 10% (v/v) acetonitrile also provided good resolution.
采用毛细管电泳法，以 α-、β-和 γ-环糊精为手性选择剂，以 20mmol/L 浓度的磷酸盐、pH2.5 为背景电解质，对手性拆分了 7 种 Tröger's 碱衍生物（TBs）。分别考察了手性选择剂（α-、β-和 γ-环糊精）浓度（0-50mmol/L）和电解质中有机溶剂（乙腈，0-25%（v/v））用量对拆分的影响。结果表明，所研究的各种变量，如手性选择剂的类型、浓度以及加入的有机溶剂的量，对研究的 TBs 的对映体拆分有显著影响。对于大多数分离的 TBs，最佳结果是在背景电解质中使用 5mmol/L 的 β-环糊精，并添加 5-15%（v/v）的乙腈。对于研究的两个最小的 TBs，在背景电解质中添加 10%（v/v）的乙腈的 γ-环糊精也能提供良好的分辨率。
