开发一种针对 mpb64 基因的环介导等温扩增检测方法，用于诊断眼内结核。
Development of a loop-mediated isothermal amplification assay targeting the mpb64 gene for diagnosis of intraocular tuberculosis.
机构信息
Ocular Microbiology Service.
出版信息
J Clin Microbiol. 2013 Nov;51(11):3839-40. doi: 10.1128/JCM.01386-13. Epub 2013 Aug 21.
Abstract
A loop-mediated isothermal amplification (LAMP) assay targeting the mpb64 gene for the diagnosis of intraocular tuberculosis was highly specific (100%), sensitive (85.7%), rapid, and easy to perform. The LAMP assay can be an alternative to conventional PCR for the diagnosis of ocular tuberculosis in resource-limited settings.
摘要
一种针对 mpb64 基因的环介导等温扩增（LAMP）检测方法，用于诊断眼内结核，具有高度特异性（100%）、敏感性（85.7%）、快速和易于操作的特点。在资源有限的情况下，LAMP 检测方法可以替代传统的 PCR 用于诊断眼部结核。
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