Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.
PLoS One. 2013 Aug 13;8(8):e71839. doi: 10.1371/journal.pone.0071839. eCollection 2013.
Previous studies by us and other groups characterized protein expression variation following long-term moderate training, whereas the effects of single bursts of exercise are less known. Making use of a proteomic approach, we investigated the effects of acute swimming exercise (ASE) on protein expression and carbonylation patterns in two hind limb muscles: the Extensor Digitorum Longus (EDL) and the Soleus, mostly composed of fast-twitch and slow-twitch fibres, respectively. Carbonylation is one of the most common oxidative modifications of proteins and a marker of oxidative stress. In fact, several studies suggest that physical activity and the consequent increase in oxygen consumption can lead to increase in reactive oxygen and nitrogen species (RONS) production, hence the interest in examining the impact of RONS on skeletal muscle proteins following ASE. Results indicate that protein expression is unaffected by ASE in both muscle types. Unexpectedly, the protein carbonylation level was reduced following ASE. In particular, the analysis found 31 and 5 spots, in Soleus and EDL muscles respectively, whose carbonylation is reduced after ASE. Lipid peroxidation levels in Soleus were markedly reduced as well. Most of the decarbonylated proteins are involved either in the regulation of muscle contractions or in the regulation of energy metabolism. A number of hypotheses may be advanced to account for such results, which will be addressed in future studies.
先前我们和其他团队的研究已经对长期中等强度训练后蛋白质表达变化的特征进行了描述，而单次运动爆发的影响则知之甚少。本研究利用蛋白质组学方法，研究了急性游泳运动（ASE）对两种后肢肌肉（伸趾长肌和比目鱼肌）中蛋白质表达和羰基化模式的影响，这两种肌肉主要由快肌纤维和慢肌纤维组成。羰基化是蛋白质最常见的氧化修饰之一，也是氧化应激的标志物。事实上，有几项研究表明，体育活动和随之而来的耗氧量增加会导致活性氧和氮物种（RONS）的产生增加，因此人们对研究 RONS 对 ASE 后骨骼肌蛋白的影响很感兴趣。结果表明，两种肌肉类型的 ASE 均不影响蛋白质表达。出乎意料的是，ASE 后蛋白质羰基化水平降低。具体来说，在比目鱼肌和伸趾长肌中，分别有 31 个和 5 个点的蛋白质羰基化水平在 ASE 后降低。比目鱼肌中的脂质过氧化水平也明显降低。大多数去羰基化的蛋白质要么参与肌肉收缩的调节，要么参与能量代谢的调节。可以提出许多假设来解释这些结果，这将在未来的研究中进一步探讨。
