Lau O W, Luk S F, Chiu T P
Department of Chemistry, Chinese University of Hong Kong, Shatin, N.T.
Analyst. 1990 May;115(5):653-5. doi: 10.1039/an9901500653.
A simple and accurate spectrophotometric method is proposed for the determination of taurine in food samples using phenol and sodium hypochlorite as reagents, which form a blue colour with taurine at room temperature and pH 10.35. Ion exchange was used to improve the selectivity of the method. Absorbance measurements were made at 630 nm and the calibration graph was linear from 0 to 180 micrograms ml-1 of taurine with a slope of 0.00242 A (p.p.m.)-1. The precision for the determination of taurine (156 micrograms ml-1) was 0.8% (n = 10). The method was applied successfully to the determination of taurine in milk products and energy drinks.
提出了一种简单准确的分光光度法，以苯酚和次氯酸钠为试剂测定食品样品中的牛磺酸，该试剂在室温及pH 10.35条件下与牛磺酸形成蓝色。采用离子交换提高该方法的选择性。在630 nm处进行吸光度测量，校准曲线在0至180微克/毫升牛磺酸范围内呈线性，斜率为0.00242 A（ppm）-1。测定牛磺酸（156微克/毫升）的精密度为0.8%（n = 10）。该方法成功应用于乳制品和能量饮料中牛磺酸的测定。
