采用牛磺酸双加氧酶测定食品和生物样品中的牛磺酸浓度的简单方法。

A simple assay of taurine concentrations in food and biological samples using taurine dioxygenase.

机构信息

Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398, Japan.

出版信息

Anal Biochem. 2012 Aug 15;427(2):121-3. doi: 10.1016/j.ab.2012.04.030. Epub 2012 May 14.

DOI:10.1016/j.ab.2012.04.030

PMID:22595347

Abstract

Taurine demonstrates various physiological functions and pharmacological actions. A successful application of taurine dioxygenase (EC 1.14.11.17) for taurine determination is described. The gene encoding taurine dioxygenase was cloned from Escherichia coli strain K-12, and the enzyme was used to determine taurine in commercially available beverages and some biological samples. The measured values obtained using the current method are close to the declared values with the precolumn derivatization ultra-performance liquid chromatography (UPLC) procedure. Taurine dioxygenase can be used for taurine determination in food control, biological research, and diagnoses based on urinary taurine concentration.

摘要

牛磺酸具有多种生理功能和药理作用。本文描述了牛磺酸双加氧酶（EC 1.14.11.17）在牛磺酸测定中的成功应用。从大肠杆菌 K-12 中克隆了编码牛磺酸双加氧酶的基因，并将该酶用于测定市售饮料和一些生物样品中的牛磺酸。使用当前方法获得的测量值与采用柱前衍生超高效液相色谱（UPLC）程序获得的声明值非常接近。牛磺酸双加氧酶可用于食品控制、生物研究以及基于尿牛磺酸浓度的诊断中的牛磺酸测定。