肺组织中酸性结构糖蛋白的分离与特性分析
Isolation and characterization of acidic structural glycoproteins in pulmonary tissues.
作者信息
Francis G, Thomas J
出版信息
Biochem J. 1975 Feb;145(2):299-304. doi: 10.1042/bj1450299.
Abstract
- Extraction of the pleural and parenchymal regions of bovine lungs with salt and organic solvents gave powders that contained glycoproteins in addition to collagen and elastin. The contents of these glycoproteins (g/100 of powder) was 4% in pleura and 15.5% in parenchyma. 2. Attempts were made to purify these glycoproteins fromboth tissues by various methods. 3. Both tissues contained heterogeneous mixtures that were similar solubilities, had similar amino acid and carbohydrate compostions. 4. The compostions of the pulmonary glycoproteins resembled those isolated from other connective tissues by other workers.
摘要
- 用盐和有机溶剂提取牛肺的胸膜和实质区域，得到的粉末除了含有胶原蛋白和弹性蛋白外，还含有糖蛋白。这些糖蛋白的含量（每100克粉末中的克数）在胸膜中为4%，在实质中为15.5%。2. 尝试通过各种方法从这两种组织中纯化这些糖蛋白。3. 两种组织都含有溶解度相似、氨基酸和碳水化合物组成相似的异质混合物。4. 肺糖蛋白的组成与其他研究者从其他结缔组织中分离出的糖蛋白相似。
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