Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology , Meilong Road 130, Shanghai 200237, China.
ACS Appl Mater Interfaces. 2013 Sep 11;5(17):8278-82. doi: 10.1021/am402429n. Epub 2013 Aug 26.
In this study, the fluorescence of Hoechst dyes is significantly lit up by addition of our designed MB probe, forming a complex of "molecular beacon"-hosted Hoechst dyes (HMB). Combined with this property, a novel graphene oxide (GO)-based fluorescent "on/off" switch was developed to visually follow bioassay utilizing HMB as signal indicators and GO as an excellent energy acceptor to efficiently quench the fluorescence of HMB in a label-easy format. We have demonstrated its application for label-easy fluorescence "turn on" detection of sequence-specific DNA and "turn off" detection of exonuclease with sensitivity and selectivity in a single step in homogeneous solution. Compared to traditional molecular beacons, the proposed design is cost-effective and simple to prepare without fluorescence labeling or chemical modification.
在这项研究中，通过添加我们设计的 MB 探针，Hoechst 染料的荧光显著增强，形成了“分子信标”负载的 Hoechst 染料（HMB）复合物。结合这一特性，开发了一种新型基于氧化石墨烯（GO）的荧光“开/关”开关，以利用 HMB 作为信号指示剂和 GO 作为优异的能量受体，以标签简便的格式可视化跟踪生物测定，从而有效地猝灭 HMB 的荧光。我们已经证明了其在均相溶液中单步进行序列特异性 DNA 的标签简便荧光“开启”检测和外切酶的“关闭”检测中的应用，具有灵敏度和选择性。与传统的分子信标相比，所提出的设计具有成本效益，并且简单易用，无需荧光标记或化学修饰。
