Department of Medicine and Epidemiology, University of California, Davis, CA 95616, United States.
Comp Immunol Microbiol Infect Dis. 2013 Dec;36(6):591-8. doi: 10.1016/j.cimid.2013.07.002. Epub 2013 Aug 19.
Amphibians are experiencing global declines due in part to the infectious disease chytridiomycosis. Some symbiotic bacteria residents on frog skin have been shown to inhibit the growth of Batrachochytrium dendrobatitis (Bd) but few studies have attempted to fully describe the resident bacterial flora of frog skin. We cultured and sequenced 130 bacterial isolates from frogs collected from the California Klamath Range, recovering predominantly Gram-negative bacteria from 20 higher order taxa and 31 genera. There were also a large number of unclassifiable isolates. Forty-three isolates were assessed for their ability to inhibit the growth of Bd in vitro; of these, two had strong and three had slight anti-Bd activity. We suggest that many bacterial species may play a secondary role in Bd resistance, acting synergistically with inhibitory species. Future research is required in order to characterize these interactions. Understanding the relationships between bacterial strains may be important in predicting and managing the effects of future anti-Bd treatments such as antimicrobial compounds or probiotic bacteria.
两栖动物正经历着全球性的衰退，部分原因是传染性疾病蛙壶菌病。一些栖息在青蛙皮肤上的共生细菌已被证明可以抑制蛙壶菌（Bd）的生长，但很少有研究试图全面描述青蛙皮肤的常驻细菌菌群。我们从加利福尼亚克拉马斯山脉采集的青蛙中培养并测序了 130 个细菌分离株，从 20 个高级分类群和 31 个属中恢复了主要的革兰氏阴性菌。还有大量不可分类的分离株。我们评估了 43 个分离株抑制 Bd 在体外生长的能力；其中，有两个具有强烈的抗 Bd 活性，有三个具有轻微的抗 Bd 活性。我们认为，许多细菌物种可能在 Bd 抗性中起次要作用，与抑制性物种协同作用。为了描述这些相互作用，需要进行未来的研究。了解细菌菌株之间的关系可能对预测和管理未来的抗 Bd 治疗（如抗菌化合物或益生菌）的效果很重要。
