Department of Medical Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Ultrasound Med Biol. 2013 Nov;39(11):2158-65. doi: 10.1016/j.ultrasmedbio.2013.05.015. Epub 2013 Aug 19.
We determined the ability of contrast-enhanced ultrasound (CEUS) using perflubutane microbubbles to diagnose liver fibrosis and cirrhosis in rats using histology as the reference standard. Fibrosis was induced by oral administration of carbon tetrachloride to 32 Wistar rats. Features with baseline ultrasound (US) and enhancement level of liver and spleen with CEUS were obtained. In the post-vascular phase of CEUS, images of normal livers (n = 5) were significantly brighter than images of fibrotic (n = 6) and cirrhotic livers (n = 13) by quantitative analysis (all p < 0.05). The contrast between livers and spleens in rats with cirrhosis was quantitatively greater than that in normal rats and rats with fibrosis (all p < 0.05). Compared with US, CEUS improved sensitivity from 63% to 84% and accuracy from 71% to 88%. Specificity was 100% for both. The increased value of CEUS in diagnosing liver fibrosis and cirrhosis in rats supports its evaluation in clinical trials.
我们使用全氟丁烷微泡的对比增强超声（CEUS）来确定其诊断纤维化和肝硬化的能力，以组织学作为参考标准。纤维化是通过给予 32 只 Wistar 大鼠口服四氯化碳来诱导的。获取基线超声（US）和肝脏及脾脏 CEUS 增强水平的特征。在 CEUS 的血管后相，正常肝脏（n=5）的图像通过定量分析明显比纤维化（n=6）和肝硬化（n=13）肝脏的图像更亮（均 p<0.05）。肝硬化大鼠的肝脏和脾脏之间的对比度在定量上大于正常大鼠和纤维化大鼠（均 p<0.05）。与 US 相比，CEUS 将灵敏度从 63%提高到 84%，将准确性从 71%提高到 88%。特异性均为 100%。CEUS 在诊断大鼠肝纤维化和肝硬化方面的增值支持其在临床试验中的评估。
