Department of Veterinary Medical Science, University of Bologna, Ozzano dell'Emilia, Bologna, Italy; Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Res Vet Sci. 2013 Dec;95(3):846-53. doi: 10.1016/j.rvsc.2013.07.028. Epub 2013 Aug 6.
The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker. Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36 ± 12% and 24 ± 5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3 ± 3%, 21 ± 5% and 6 ± 2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR. These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.
肠神经元的神经化学在小型实验啮齿动物（豚鼠、小鼠、大鼠）的不同物种中存在差异。在这项研究中，我们对 bankvole（Myodes glareolus）回肠肌间神经丛（MP）神经元细胞和纤维的表型进行了特征描述，bankvole 是一种常见的啮齿动物，分布于欧洲和北亚，也被用于朊病毒实验传播研究。我们测试了六种神经元标志物：胆碱乙酰转移酶（ChAT）、神经元型一氧化氮合酶（nNOS）、钙结合蛋白（CALB）、降钙素基因相关肽（CGRP）和 P 物质（SP），以及 HuC/D 作为全神经元标志物。表达 ChAT-和 nNOS-免疫反应性（IR）的神经元分别为 36±12%和 24±5%。表达 CGRP-、SP-和 CALB-IR 的神经元分别为 3±3%、21±5%和 6±2%。因此，bankvole MPs 与表达 CGRP-、SP-和 CALB-IR 的小鼠 MPs 一致存在差异。这些数据可能有助于确定来自 bankvole 的回肠 MPs 中神经元群体对朊病毒的易感性，以及它们在口服实验性朊病毒挑战后的形态和功能改变。
