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肝线粒体后上清液将L-[1-¹⁴C]亮氨酸掺入蛋白质的过程：预孵育的烟酰胺腺嘌呤二核苷酸磷酸和4-二甲基氨基-3'-甲基偶氮苯的相反作用。

Incorporation of L-[1-14C]leucine into protein by liver postmitochondrial supernatant: opposing effects of preincubated nicotinamide-adenine dinucleotide phosphate and 4-dimethylamino-3'-methylazobenzene.

作者信息

Madsen N P, Labuc J E

出版信息

Biochem J. 1975 Feb;146(2):505-7. doi: 10.1042/bj1460505.

DOI:10.1042/bj1460505
PMID:239694
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1165331/
Abstract

Combination of preincubated drug-metabolizing medium containing NADP+ with a cell-free protein-synthesizing system resulted in marked stimulation of incorporation of L-[1-14C]leucine into protein. Addition of 4-dimethylamino-3'-methylazobenzene, present and previously preincubated in the drug-metabolizing medium, decreased this effect.

摘要

含有NADP⁺的预孵育药物代谢介质与无细胞蛋白质合成系统相结合，导致L-[1-¹⁴C]亮氨酸掺入蛋白质的过程受到显著刺激。在药物代谢介质中存在并预先孵育的4-二甲基氨基-3'-甲基偶氮苯的添加降低了这种效应。

相似文献

1
Incorporation of L-[1-14C]leucine into protein by liver postmitochondrial supernatant: opposing effects of preincubated nicotinamide-adenine dinucleotide phosphate and 4-dimethylamino-3'-methylazobenzene.肝线粒体后上清液将L-[1-¹⁴C]亮氨酸掺入蛋白质的过程：预孵育的烟酰胺腺嘌呤二核苷酸磷酸和4-二甲基氨基-3'-甲基偶氮苯的相反作用。
Biochem J. 1975 Feb;146(2):505-7. doi: 10.1042/bj1460505.
2
Inhibition by 3'-methyl-4-dimethylamino-azobenzene of in vitro cell-free protein synthesis: possible involvement of an electrophilic metabolite.3'-甲基-4-二甲基氨基偶氮苯对体外无细胞蛋白质合成的抑制作用：亲电代谢物可能的参与。
Biochem Pharmacol. 1977 May 15;26(10):929-34. doi: 10.1016/0006-2952(77)90468-3.
3
The concentration and biosynthesis of nicotinamide nucleotides in the livers of rats treated with carcinogens.用致癌物处理的大鼠肝脏中烟酰胺核苷酸的浓度和生物合成。
Biochem J. 1966 Feb;98(2):546-56. doi: 10.1042/bj0980546.
4
[Effect of some azo compounds on 1-leucine incorporation by the liver microsome system].[某些偶氮化合物对肝微粒体系统掺入亮氨酸的影响]
C R Acad Hebd Seances Acad Sci D. 1965 May 3;260(18):4871-3.
5
In vitro incorporation of (1- 14 C)leucine into rat liver microsomal protein after dietary supplementation with 3'methyl-4-dimethylaminoazobenzene, chloramphenicol or both compounds.用3'-甲基-4-二甲基氨基偶氮苯、氯霉素或这两种化合物进行膳食补充后，（1-¹⁴C）亮氨酸在大鼠肝微粒体蛋白中的体外掺入情况。
Chem Biol Interact. 1972 Feb;4(3):209-17. doi: 10.1016/0009-2797(72)90015-4.
6
[Influence of diethylnitrosamine, carbon tetrachloride, butter yellow and cyclophosphamide on amino acid incorporation into fractions of liver homogenates after metabolic activation in vitro. Possibilities for the detection of hepatotoxic effects].[二乙基亚硝胺、四氯化碳、奶油黄和环磷酰胺对体外代谢活化后氨基酸掺入肝匀浆各组分的影响。检测肝毒性作用的可能性]
Arch Toxikol. 1969;25(2):169-82.
7
In vivo incorporation of (1- 14 C)leucine into rat liver and serum protein after dietary supplementation with 3'-methyl-4-dimethylaminoazobenzene, chloramphenicol or both compounds.在大鼠饮食中补充3'-甲基-4-二甲基氨基偶氮苯、氯霉素或这两种化合物后，（1-¹⁴C）亮氨酸在大鼠肝脏和血清蛋白中的体内掺入情况。
Chem Biol Interact. 1972 Jan;4(2):103-12. doi: 10.1016/0009-2797(72)90004-x.
8
[Role of lysosome permeability disorders in the mechanism of the low-temperature inhibition of protein biosynthesis in the rat liver].[溶酶体通透性紊乱在大鼠肝脏低温抑制蛋白质生物合成机制中的作用]
Biull Eksp Biol Med. 1981 Jun;91(6):675-6.
9
[Effect of cryoprotectants on protein-synthesizing activity of cell-free system from rat liver].
Ukr Biokhim Zh. 1977 Jul-Aug;49(4):39-42.
10
Effect of insulin on albumin production and incorporation of 14C-leucine into proteins in isolated parenchymal liver cells from normal rats.胰岛素对正常大鼠分离的肝实质细胞中白蛋白生成及14C-亮氨酸掺入蛋白质的影响。
Acta Physiol Scand. 1975 Jun;94(2):236-43. doi: 10.1111/j.1748-1716.1975.tb05883.x.

本文引用的文献

1
Use of toluene/triton X-100 scintillation mixture for counting C14-protein radioactivity.使用甲苯/曲拉通X-100闪烁混合物计数C14蛋白放射性。
Anal Biochem. 1969 Jun;29(3):542-4. doi: 10.1016/0003-2697(69)90341-8.
2
Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimethable and ribosome fractions of rat liver after a single injection of lebaled orotic acid.单次注射标记乳清酸后，大鼠肝脏核、非沉降及核糖体组分中酸溶性部分与RNA部分之间放射性的分布情况。
Biochim Biophys Acta. 1968 Aug 23;166(1):48-57. doi: 10.1016/0005-2787(68)90489-9.
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In vitro amino acid incorporation by the post-mitochondrial supernatant from rat liver.
Biochim Biophys Acta. 1971 Dec 30;254(3):468-77. doi: 10.1016/0005-2787(71)90881-1.
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Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues.分泌性和非分泌性组织的膜结合核糖体和游离核糖体的蛋白质合成。
Biochem J. 1971 Feb;121(4):683-94. doi: 10.1042/bj1210683.
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Carcinogenesis by chemicals: an overview--G. H. A. Clowes memorial lecture.化学物质致癌作用概述——G. H. A. 克劳斯纪念讲座
Cancer Res. 1970 Mar;30(3):559-76.
6
Protein synthesis in a postmitochondrial supernatant system from rat liver. An effect of diabetes at the level of peptide chain initiation.大鼠肝脏线粒体后上清液系统中的蛋白质合成。糖尿病在肽链起始水平的影响。
FEBS Lett. 1973 Sep 1;35(1):169-72. doi: 10.1016/0014-5793(73)80603-9.
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Comparative studies on two reaction center preparations from Rhodopseudomonas speheroides Y.球形红假单胞菌Y的两种反应中心制剂的比较研究
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