硫代取代脂肪酸对线粒体和过氧化物酶体β-氧化的影响。体内和体外研究。
Effects of thia-substituted fatty acids on mitochondrial and peroxisomal beta-oxidation. Studies in vivo and in vitro.
作者信息
Hovik R, Osmundsen H, Berge R, Aarsland A, Bergseth S, Bremer J
机构信息
Department of Physiology and Biochemistry, Dental School, University of Oslo, Norway.
出版信息
Biochem J. 1990 Aug 15;270(1):167-73. doi: 10.1042/bj2700167.
Abstract
- The effects of 3-, 4- and 5-thia-substituted fatty acids on mitochondrial and peroxisomal beta-oxidation have been investigated. When the sulphur atom is in the 4-position, the resulting thia-substituted fatty acid becomes a powerful inhibitor of beta-oxidation. 2. This inhibition cannot be explained in terms of simple competitive inhibition, a phenomenon which characterizes the inhibitory effects of 3- and 5-thia-substituted fatty acids. The inhibitory sites for 4-thia-substituted fatty acids are most likely to be the acyl-CoA dehydrogenase in mitochondria and the acyl-CoA oxidase in peroxisomes. 3. The inhibitory effect of 4-thia-substituted fatty acids is expressed both in vitro and in vivo. The effect in vitro is instantaneous, with up to 95% inhibition of palmitoylcarnitine oxidation. The effect in vivo, in contrast, is dose-dependent and increases with duration of treatment. 4. Pretreatment of rats with a 3-thia-substituted fatty acid rendered mitochondrial beta-oxidation less sensitive to inhibition by 4-thia-substituted fatty acids.
摘要
- 研究了3-、4-和5-硫代取代脂肪酸对线粒体和过氧化物酶体β-氧化的影响。当硫原子处于4位时，生成的硫代取代脂肪酸成为β-氧化的强力抑制剂。2. 这种抑制作用不能用简单的竞争性抑制来解释，而3-和5-硫代取代脂肪酸的抑制作用具有这种特征。4-硫代取代脂肪酸的抑制位点最有可能是线粒体中的酰基辅酶A脱氢酶和过氧化物酶体中的酰基辅酶A氧化酶。3. 4-硫代取代脂肪酸的抑制作用在体外和体内均有表现。体外作用是即时的，对棕榈酰肉碱氧化的抑制率高达95%。相比之下，体内作用是剂量依赖性的，且随治疗持续时间增加。4. 用3-硫代取代脂肪酸预处理大鼠，使线粒体β-氧化对4-硫代取代脂肪酸的抑制作用不那么敏感。
相似文献
1
Effects of thia-substituted fatty acids on mitochondrial and peroxisomal beta-oxidation. Studies in vivo and in vitro.硫代取代脂肪酸对线粒体和过氧化物酶体β-氧化的影响。体内和体外研究。
Biochem J. 1990 Aug 15;270(1):167-73. doi: 10.1042/bj2700167.
2
Comparative effects of oxygen and sulfur-substituted fatty acids on serum lipids and mitochondrial and peroxisomal fatty acid oxidation in rat.氧代和硫代脂肪酸对大鼠血脂及线粒体和过氧化物酶体脂肪酸氧化的比较作用
Biochem Pharmacol. 1992 Feb 18;43(4):815-22. doi: 10.1016/0006-2952(92)90248-h.
3
Peroxisomal fatty acid oxidation and inhibitors of the mitochondrial carnitine palmitoyltransferase I in isolated rat hepatocytes.分离的大鼠肝细胞中过氧化物酶体脂肪酸氧化及线粒体肉碱棕榈酰转移酶I抑制剂
Biochem J. 1992 Jan 15;281 ( Pt 2)(Pt 2):561-7. doi: 10.1042/bj2810561.
4
Alkylthio acetic acids (3-thia fatty acids)--a new group of non-beta-oxidizable peroxisome-inducing fatty acid analogues--II. Dose-response studies on hepatic peroxisomal- and mitochondrial changes and long-chain fatty acid metabolizing enzymes in rats.烷硫基乙酸（3-硫代脂肪酸）——一类新型的非β-氧化可诱导过氧化物酶体的脂肪酸类似物——II. 对大鼠肝脏过氧化物酶体和线粒体变化以及长链脂肪酸代谢酶的剂量反应研究
Biochem Pharmacol. 1989 Nov 15;38(22):3969-79. doi: 10.1016/0006-2952(89)90676-x.
5
Inhibition of hepatic fatty acid oxidation at carnitine palmitoyltransferase I by the peroxisome proliferator 2-hydroxy-3-propyl-4-[6-(tetrazol-5-yl) hexyloxy]acetophenone.过氧化物酶体增殖物2-羟基-3-丙基-4-[6-(四氮唑-5-基)己氧基]苯乙酮对肉碱棕榈酰转移酶I处肝脂肪酸氧化的抑制作用
Biochem J. 1988 Jun 1;252(2):409-14. doi: 10.1042/bj2520409.
6
Fatty acid metabolism in liver of rats treated with hypolipidemic sulphur-substituted fatty acid analogues.用降血脂硫代取代脂肪酸类似物处理的大鼠肝脏中的脂肪酸代谢
Biochim Biophys Acta. 1990 May 22;1044(2):211-21. doi: 10.1016/0005-2760(90)90305-h.
7
8
9
Regulation of pathways of extramitochondrial fatty acid oxidation and liver fatty acid-binding protein by long-chain monocarboxylic fatty acids in hepatocytes. Effect of inhibition of carnitine palmitoyltransferase I.长链单羧酸脂肪酸对肝细胞线粒体外脂肪酸氧化途径和肝脏脂肪酸结合蛋白的调节作用。肉碱棕榈酰转移酶I抑制的影响
J Biol Chem. 1993 Dec 25;268(36):26866-71.
10
Early modulation of genes encoding peroxisomal and mitochondrial beta-oxidation enzymes by 3-thia fatty acids.3-硫代脂肪酸对编码过氧化物酶体和线粒体β-氧化酶的基因的早期调控
Biochem Pharmacol. 1998 Dec 15;56(12):1571-82. doi: 10.1016/s0006-2952(98)00193-2.
引用本文的文献
1
Associations between fatty acid oxidation, hepatic mitochondrial function, and plasma acylcarnitine levels in mice.小鼠脂肪酸氧化、肝脏线粒体功能与血浆酰基肉碱水平之间的关联。
Nutr Metab (Lond). 2018 Jan 29;15:10. doi: 10.1186/s12986-018-0241-7. eCollection 2018.
2
Thia fatty acids with the sulfur atom in even or odd positions have opposite effects on fatty acid catabolism.硫原子位于偶数或奇数位置的这些脂肪酸对脂肪酸分解代谢具有相反的作用。
Lipids. 2006 Feb;41(2):169-77. doi: 10.1007/s11745-006-5085-7.
3
Synthesis and antifungal properties of alpha-methoxy and alpha-hydroxyl substituted 4-thiatetradecanoic acids.α-甲氧基和α-羟基取代的4-硫代十四烷酸的合成及其抗真菌特性
Chem Phys Lipids. 2007 Nov;150(1):82-8. doi: 10.1016/j.chemphyslip.2007.06.218. Epub 2007 Jun 28.
4
Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors alpha and delta.降血脂药物、多不饱和脂肪酸和类二十烷酸是过氧化物酶体增殖物激活受体α和δ的配体。
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4312-7. doi: 10.1073/pnas.94.9.4312.
5
Effects of tetradecylthiopropionic acid and tetradecylthioacrylic acid on rat liver lipid metabolism.十四烷基硫代丙酸和十四烷基硫代丙烯酸对大鼠肝脏脂质代谢的影响。
Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):591-7. doi: 10.1042/bj3050591.
6
The metabolism of tetradecylthiopropionic acid, a 4-thia stearic acid, in the rat. In vivo and in vitro studies.大鼠体内4-硫代硬脂酸——十四烷基硫代丙酸的代谢：体内和体外研究
Biochem J. 1992 Sep 15;286 ( Pt 3)(Pt 3):879-87. doi: 10.1042/bj2860879.
本文引用的文献
1
Induction of acyl-CoA dehydrogenases and electron transfer flavoprotein and their roles in fatty acid oxidation in rat liver mitochondria.大鼠肝脏线粒体中酰基辅酶A脱氢酶和电子传递黄素蛋白的诱导及其在脂肪酸氧化中的作用。
J Biochem. 1981 Dec;90(6):1751-6. doi: 10.1093/oxfordjournals.jbchem.a133652.
2
The effect of fasting on the activity of liver carnitine palmitoyltransferase and its inhibition by malonyl-CoA.禁食对肝脏肉碱棕榈酰转移酶活性的影响及其被丙二酰辅酶A的抑制作用。
Biochim Biophys Acta. 1981 Sep 24;665(3):628-31. doi: 10.1016/0005-2760(81)90282-4.
3
A role for 2,4-enoyl-CoA reductase in mitochondrial beta-oxidation of polyunsaturated fatty acids. Effects of treatment with clofibrate on oxidation of polyunsaturated acylcarnitines by isolated rat liver mitochondria.2,4-烯酰辅酶A还原酶在多不饱和脂肪酸线粒体β-氧化中的作用。氯贝丁酯处理对分离的大鼠肝脏线粒体氧化多不饱和酰基肉碱的影响。
Biochem J. 1982 Dec 15;208(3):749-57. doi: 10.1042/bj2080749.
4
Effects of high-fat diets on hepatic fatty acid oxidation in the rat. Isolation of rat liver peroxisomes by vertical-rotor centrifugation by using a self-generated, iso-osmotic, Percoll gradient.高脂饮食对大鼠肝脏脂肪酸氧化的影响。通过使用自生等渗Percoll梯度，采用垂直转子离心法分离大鼠肝脏过氧化物酶体。
Biochem J. 1981 Apr 15;196(1):149-59. doi: 10.1042/bj1960149.
5
The activity of the different carnitine acyltransferases in the liver of clofibrate-fed rats.氯贝丁酯喂养大鼠肝脏中不同肉碱酰基转移酶的活性。
Biochim Biophys Acta. 1972 Nov 30;280(3):434-9. doi: 10.1016/0005-2760(72)90249-4.
6
Inhibitory effects of some long-chain unsaturated fatty acids on mitochondrial beta-oxidation. Effects of streptozotocin-induced diabetes on mitochondrial beta-oxidation of polyunsaturated fatty acids.一些长链不饱和脂肪酸对线粒体β-氧化的抑制作用。链脲佐菌素诱导的糖尿病对多不饱和脂肪酸线粒体β-氧化的影响。
Biochem J. 1985 Sep 1;230(2):329-37. doi: 10.1042/bj2300329.
7
Protection of rats by clofibrate against the hypoglycaemic and toxic effects of hypoglycin and pent-4-enoate. An ultrastructural and biochemical study.氯贝丁酯对大鼠低血糖素和戊-4-烯酸的降血糖及毒性作用的保护作用。一项超微结构和生化研究。
Biochem J. 1985 Jul 15;229(2):387-97. doi: 10.1042/bj2290387.
8
Peroxisomal beta-oxidation of long-chain fatty acids possessing different extents of unsaturation.具有不同不饱和程度的长链脂肪酸的过氧化物酶体β-氧化
Biochem J. 1987 Nov 1;247(3):531-5. doi: 10.1042/bj2470531.
9
The reductive half-reaction in Acyl-CoA dehydrogenase from pig kidney: studies with thiaoctanoyl-CoA and oxaoctanoyl-CoA analogues.猪肾酰基辅酶A脱氢酶中的还原半反应：硫辛酸辛酰辅酶A和氧辛酸辛酰辅酶A类似物的研究
Biochemistry. 1988 Jul 12;27(14):5089-95. doi: 10.1021/bi00414a021.
10
Alkylthioacetic acid (3-thia fatty acids)--a new group of non-beta-oxidizable, peroxisome-inducing fatty acid analogues. I. A study on the structural requirements for proliferation of peroxisomes and mitochondria in rat liver.烷硫基乙酸（3-硫代脂肪酸）——一类新型的不可进行β氧化、可诱导过氧化物酶体的脂肪酸类似物。I. 大鼠肝脏中过氧化物酶体和线粒体增殖的结构要求研究
Biochim Biophys Acta. 1989 Aug 22;1004(3):345-56. doi: 10.1016/0005-2760(89)90083-0.
Zotero 插件