Sanches Eduardo G, Oliveira Idili R, Serralheiro Pedro C Da Silva, Cerqueira Vinicius R
Núcleo de Pesquisa e Desenvolvimento do Litoral Norte, Instituto de Pesca/APTA/SAA, 11680-000 Ubatuba, SP, Brasil.
An Acad Bras Cienc. 2013 Sep;85(3):1083-1091. doi: 10.1590/S0001-37652013005000047.
This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2) , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10%) and three cooling rates ( -90; -60 and -30°C.min-1) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P < 0.05) was achieved by combining extender C ( pH 8.2) with DMSO ( 10%) and cooling rate of -60°C.min-1 ( P < 0.05) . The use of cryopreserved sperm presented fertilization rates higher than 59% validating the present protocol for mutton snapper.
本研究旨在制定一种笛鲷精液冷冻保存方案。通过析因实验分析了三种稀释液（pH 6.1、7.8和8.2）、两种二甲亚砜（DMSO）浓度（5%和10%）以及三种降温速率（-90、-60和-30°C·min-1）对精子活力和活力时间的相互作用。采集了30尾自然环境中的笛鲷（1261±449克），饲养于浮动网箱中。精液采用低温细管在氮气中冷冻，随后转移至液氮中。通过受精试验评估冷冻精子的活力。将稀释液C（pH 8.2）与DMSO（10%）以及降温速率-60°C·min-1相结合时，精子活力和活力时间最高（P<0.05）。使用冷冻精子的受精率高于59%，验证了本笛鲷精液冷冻保存方案的有效性。
