Sanches E G, Oliveira I R, Serralheiro P C S, Cerqueira V R
Agência Paulista de Tecnologia dos Agronegócios, Secretaria da Agricultura e Abastecimento, Ubatuba, SP, BR.
Departamento de Aquicultura, Universidade Federal de Santa Catarina, Florianópolis, SC, BR.
Braz J Biol. 2015 Aug;75(3):662-9. doi: 10.1590/1519-6984.20613. Epub 2015 Aug 25.
This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C -min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.
本研究旨在开发并评估紫红笛鲷精液冷冻保存方案。首先，评估精子活力率、活力时间、密度和血细胞比容，以表征紫红笛鲷的精子质量。分析了三种具有不同离子组成和pH值的稀释液与七种浓度的冷冻保护剂二甲基亚砜（0；2.5；5.0；7.5；10.0；12.5和15.0%）、五种降温速率（110、90、60、45和30°C·min）、九种平衡时间（1；2.5；5；10；15；20；25；30和60分钟）以及五种稀释比例（1:1；1:3；1:6；1:10和1:20）对精子活力率和活力时间的影响。进行受精试验以评估冷冻保存精子的活力。通过将pH值为8.2的稀释液与10%浓度的二甲基亚砜、60°C·min的降温速率、1分钟的平衡时间和1:3（v/v）的稀释比例相结合，获得了较高的精子活力率和活力时间（P<0.05）。使用冷冻保存的精子受精率>60%，验证了当前紫红笛鲷的方案。紫红笛鲷的冷冻保存精子是一种可行的选择，能够保持适当的精子活力。
