De Oliveira Flavia, Maifrino Laura B M, De Jesus Gustavo P P, Carvalho Juliana G, Marchon Cláudia, Ribeiro Daniel A
Departamento de Biociências, Universidade Federal de São Paulo, Campus Baixada Santista, 11060-001 Santos SP, Brasil.
An Acad Bras Cienc. 2013 Sep;85(3):1157-64. doi: 10.1590/S0001-37652013005000046.
Estrogen deprivation in postmenopausal women increases cardiovascular risk. Cardiovascular risk as a result of atherosclerosis is able to induce an inflammatory disease as far as cyclooxygenase-2 ( COX-2) expression. The purpose of the study was to investigate the role of COX-2 on exercise training in female mice low-density lipoprotein receptor knockout ( LDL-KO) with or without ovariectomy. A total of 15 female C57BL/6 mice and 15 female LDL-KO mice were distributed into 6 groups: sedentary control, sedentary control ovariectomized, trained control ovariectomized, LDL-KO sedentary, LDL-KO sedentary ovariectomized and LDL-KO trained ovariectomized. The ascending part of the aorta was stained with H&E and COX-2 expression was assessed by immunohistochemistry. Results revealed that ovariectomy as well as exercise training were not able to induce histopathological changes in mouse aorta for all groups investigated. LDL-KO mice demonstrated plaque containing cholesterol clefts, foamy histiocytes and mild inflammatory process for all groups indistinctly. Ovariectomy induced a strong immunoexpression in atherosclerosis lesion of LDL-KO mice. Nevertheless, a down-regulation of COX-2 expression was detected in LDL-KO trained ovariectomized when compared to LDL-KO sedentary. Our results are consistent with the notion that exercise training is able to modulate COX-2 expression in LDL-KO mice as a result of COX-2 down-regulation.
绝经后女性雌激素缺乏会增加心血管疾病风险。动脉粥样硬化导致的心血管疾病风险能够引发一种炎症性疾病，这与环氧合酶-2（COX-2）的表达有关。本研究的目的是探讨COX-2在有或无卵巢切除的雌性低密度脂蛋白受体敲除（LDL-KO）小鼠运动训练中的作用。总共15只雌性C57BL/6小鼠和15只雌性LDL-KO小鼠被分为6组：久坐对照组、久坐卵巢切除对照组、训练卵巢切除对照组、LDL-KO久坐组、LDL-KO久坐卵巢切除组和LDL-KO训练卵巢切除组。用苏木精和伊红对主动脉升部进行染色，并通过免疫组织化学评估COX-2的表达。结果显示，对于所有研究组，卵巢切除以及运动训练均未在小鼠主动脉中诱导组织病理学变化。LDL-KO小鼠的所有组均表现出含有胆固醇裂隙、泡沫状组织细胞和轻度炎症过程的斑块。卵巢切除在LDL-KO小鼠的动脉粥样硬化病变中诱导了强烈的免疫表达。然而，与LDL-KO久坐组相比，在LDL-KO训练卵巢切除组中检测到COX-2表达下调。我们的结果与以下观点一致，即运动训练能够通过COX-2下调来调节LDL-KO小鼠中的COX-2表达。
