Hu Xinkun, Dai Shoufen, Pu Zhien, Liu Dengcai, Pu Zongjun, Jiang Jiaqi, Wei Yuming, Wu Bihua, Lan Xiujin, Zheng Youliang, Yan Zehong
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
J Genet. 2013;92(2):241-5. doi: 10.1007/s12041-013-0258-7.
Triticum turgidum ssp. dicoccon PI94668 and PI349045 were identified as containing null alleles at Glu-A1 and Glu-B1 loci in previous investigation. Sequencing of the respective HMW-GS genes Ax, Bx, Ay and By in both accessions indicated equal DNA lengths with gene silencing caused by 1 to 4 in-frame stop codon(s) in the open reading frames. Six synthetic hexaploid wheat lines were produced by crossing PI94668 or PI349045 with six Aegilops tauschii by spontaneous chromosome doubling of unreduced gametes. As expected, these amphiploids had three different HMW-GS: Dx 3.1(t) + Dy11(*t), Dx2.1(t) +10(t) and Dx2(t) +Dy12(t) in Glu-D1 but double nulls in Glu-A1 and Glu-B1. Quality tests showed that most quality parameters in two T. turgidum ssp. dicoccon parents were very low due to the lack of HMW-GSs. However, incorporation of HMW-GS from Ae. tauschii in six synthetic hexaploid wheat lines significantly increased most quality related parameters. The potential values of these wheat lines in improving the quality of wheat are discussed.
在之前的研究中，已鉴定出圆锥小麦PI94668和PI349045在Glu - A1和Glu - B1位点含有无效等位基因。对这两个材料中各自的高分子量谷蛋白亚基（HMW - GS）基因Ax、Bx、Ay和By进行测序，结果表明其DNA长度相等，且开放阅读框中存在1至4个框内终止密码子导致基因沉默。通过将PI94668或PI349045与6个节节麦杂交，并对未减数配子进行自发染色体加倍，培育出了6个人工合成六倍体小麦品系。正如预期的那样，这些双二倍体在Glu - D1位点具有三种不同的HMW - GS：Dx 3.1(t) + Dy11(*t)、Dx2.1(t) +10(t)和Dx2(t) +Dy12(t)，但在Glu - A1和Glu - B1位点为双无效。品质测试表明，由于缺乏HMW - GS，两个圆锥小麦亲本的大多数品质参数都非常低。然而，在6个人工合成六倍体小麦品系中导入来自节节麦的HMW - GS显著提高了大多数与品质相关的参数。本文讨论了这些小麦品系在改善小麦品质方面的潜在价值。
