Plant Breeding Institute, Maris Lane, Trumpington, Cambridge, England.
Theor Appl Genet. 1981 Oct;60(4):229-36. doi: 10.1007/BF02342544.
The high-molecular-weight (HMW) subunits of glutenin from about 185 varieties were fractionated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). About 20 different, major subunits were distinguished by this technique although each variety contained, with only a few exceptions, between 3 and 5 subunits. Further inter-varietal substitution lines to those already described (Payne et al. 1980) were analysed and the results indicate that all the HMW subunits are controlled by the homoeologous group 1 chromosomes. All hexaploid varieties studied except 'NapHal' contained two major subunits controlled by chromosome 1D. Their genes were shown to be tightly linked genetically for only four different types of banding patterns were observed. The nominal molecular weights determined after fractionation in 10% polyacrylamide gels were between 110,000 and 115,000 for the larger of the two subunits and between 82,000 and 84,000 for the smaller. One quarter of the varieties contained only one major HMW subunit controlled by chromosome 1B whereas the rest had two. The chromosome 1B subunits were the most varied and nine different banding patterns were detected. All the subunits had mobilities which were intermediate between those of the two chromosome 1D-controlled subunits. Only two types of HMW subunit controlled by chromosome 1A were detected in all the varieties examined; a single variety never contained both of these subunits and 40% of varieties contained neither. The chromosome 1A-controlled subunits had slightly slower mobilities in 10% gels than the largest HMW subunit controlled by chromosome 1D. About 100 single grains were analysed from each of five different crosses of the type (F1 of variety A × variety B) × variety C. The results indicate that the genes on chromosome 1B which control the synthesis of subunits 6, 7, 13, 14 and 17 are allelic, as are the genes of the chromosome 1A-controlled subunits, 1 and 2.
来自约 185 个品种的高分子量（HMW）醇溶蛋白亚基通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）进行了分离。尽管每种品种仅含有 3 到 5 个亚基，但通过该技术区分了约 20 种不同的主要亚基。对已经描述过的（Payne 等人，1980 年）的品种间替换系进行了进一步分析，结果表明所有 HMW 亚基均由同源群 1 染色体控制。除“NapHal”外，所有研究的六倍体品种均含有由染色体 1D 控制的两个主要亚基。基因分析表明，这些基因在遗传上紧密连锁，仅观察到四种不同的带型。在 10%聚丙烯酰胺凝胶中分级后确定的分子量，较大的两个亚基的分子量为 110,000 至 115,000，较小的亚基的分子量为 82,000 至 84,000。四分之一的品种仅含有一个由染色体 1B 控制的主要 HMW 亚基，而其余品种则含有两个。染色体 1B 亚基的变异最大，检测到 9 种不同的带型。所有亚基的迁移率均介于由染色体 1D 控制的两个亚基之间。在所研究的所有品种中，仅检测到由染色体 1A 控制的两种 HMW 亚基；单一品种从未同时含有这两个亚基，而 40%的品种均不含有。在 10%凝胶中，由染色体 1A 控制的亚基的迁移率比由染色体 1D 控制的最大 HMW 亚基略慢。从五个不同杂交类型（品种 A 的 F1 ×品种 B）×品种 C 的每个品种中分析了约 100 个单粒。结果表明，控制亚基 6、7、13、14 和 17 合成的染色体 1B 上的基因是等位的，与染色体 1A 控制的亚基 1 和 2 的基因也是等位的。
