Jing Feng, Jiao-Jiao Xu, Rin-Ming Lin, Yue-Qiu He, Shi-Chang Xu
State Key Laboratory for Biology of Plant Disease and Insect of Plant Protection, Institute of Plant Protection, Chinese Academy of Agriculture Science, No 2, West Yuan Ming Yuan Road, Beijing 100193, People's Republic of China.
J Genet. 2013;92(2):267-72. doi: 10.1007/s12041-013-0260-0.
Strubes Dickkopf is the sixth differential in the world set for wheat stripe (yellow) rust. It is very important to clarify its genetic character of resistance to stripe rust and to develop the molecular markers linked to resistance genes. The NIL Taichung 296/Strubes Dickkopf, which was obtained by Strubes Dickkopf as the gene donor and Taichung 29 as the genetic background through backcross breeding, was crossed with the recurrent parent Taichung 29, inbred, and backcrossed to obtain the F1, F2 and BC1 population. The genetic analysis of the cross Taichung 29/(Taichung 296/Strubes Dickkopf) was assessed by inoculating the rust race CYR26 at seedling stage. Bulked segregant analysis (BSA) and F2 segregation analysis were used for detecting polymorphic primers to locate the gene. The resistance of the NIL Taichung 29*6/Strubes Dickkopf to CYR26 was controlled by a single dominant gene, named YrSD. The primer pair Xbarc59 on 5B was linked to YrSD and the genetic distance between Xbarc59 and YrSD was 2.4 cM. The molecular marker Xbarc59 closely linked to the gene YrSD could be used in marker-assisted selection for resistance to stripe rust in wheat breeding programmes.
斯特鲁贝斯·迪克kopf是世界上小麦条锈病（黄色锈病）的第六个鉴别寄主。明确其抗条锈病的遗传特性并开发与抗性基因连锁的分子标记非常重要。以斯特鲁贝斯·迪克kopf为基因供体、台中29为遗传背景，通过回交育种获得的近等基因系台中296/斯特鲁贝斯·迪克kopf，与轮回亲本台中29杂交、自交并回交，获得了F1、F2和BC1群体。在苗期接种锈菌小种CYR26，对杂交组合台中29/（台中296/斯特鲁贝斯·迪克kopf）进行遗传分析。采用混合分离分析法（BSA）和F2分离分析法检测多态性引物以定位该基因。近等基因系台中29*6/斯特鲁贝斯·迪克kopf对CYR26的抗性由一个显性单基因控制，命名为YrSD。位于5B染色体上的引物对Xbarc59与YrSD连锁，Xbarc59与YrSD之间的遗传距离为2.4 cM。与基因YrSD紧密连锁的分子标记Xbarc59可用于小麦育种项目中抗条锈病的分子标记辅助选择。
