Gore M G, Iwatsubo M
Biochem J. 1975 Apr;147(1):181-4. doi: 10.1042/bj1470181.
Stopped-flow fluorescence techniques have been used to observe the formation of the binary comples of E-NADPH. At pH 7.5 there is a protein conformational change after the formation of the binary complex. This conformational change can be detected by a decrease in the fluorescence intensity of the complex at 350 nm and by an increase in its fluorescence intensity at 450 nm.
停流荧光技术已被用于观察E-NADPH二元复合物的形成。在pH 7.5时，二元复合物形成后会发生蛋白质构象变化。这种构象变化可以通过复合物在350nm处荧光强度的降低以及在450nm处荧光强度的增加来检测。
