豚鼠大脑皮层突触膜中磷脂酶A2的净活性受递质物质和假定递质物质的刺激。
The stimulation by transmitter substances and putative transmitter substances of the net activity of phospholipase A2 of synaptic membranes of cortex of guinea-pig brain.
作者信息
Gullis R J, Rowe C E
出版信息
Biochem J. 1975 May;148(2):197-208. doi: 10.1042/bj1480197.
Abstract
- The distribution of the hydrolyses of phosphatidylcholine by phospholipase A2 and phospholipase A1, and the hydrolysis of lysophosphatidylcholine by lysophospholipase, in subcellular and subsynaptosomal fractions of cerebral cortices of guinea-pig brain, was determined. 2. Noradrenaline stimulated hydrolysis by phospholipase A2 in whole synaptosomes, synaptic membranes and fractions containing synaptic vesicles. 3. Stimulation of hydrolysis by phospholipase A2 in synaptic membranes by noradrenaline was enhanced by CaCl2, and by a mixture of ATP and MgCl2. The optimum concentration of CaCl2, in the presence of ATP and MgCl2, for stimulation by 10 muM-noradrenaline was in the range 1-10muM. The optimum concentration for ATP-2MgCl2 in the presence of 1 muM-CaCl2 was in the range 0.1-1mM. 4. Hydrolysis by phospholipase A2 of synaptic membranes was also stimulated by acetylcholine, carbamoylcholine, 5-hydroxytryptamine, dopamine (3,4-dihydroxyphenethylamine), histamine, psi-aminobutyric acid, glutamic acid and aspartic acid. With appropriate concentrations of cofactors, sigmoidal dose-response curves were obtained, half-maximum stimulations being obtained with concentrations of stimulant in the range 0.1-1muM. 5. Taurine also stimulated hydrolysis of phosphatidylcholine by phospholipase A2. There were only slight stimulations with methylamine, ethylenediamine or spermidine. No stimulation was obtained with glucagon.
摘要
- 测定了豚鼠脑皮质亚细胞和突触体下部分中磷脂酶A2和磷脂酶A1对磷脂酰胆碱的水解作用，以及溶血磷脂酶对溶血磷脂酰胆碱的水解作用。2. 去甲肾上腺素刺激全突触体、突触膜和含有突触小泡的部分中磷脂酶A2的水解作用。3. CaCl2以及ATP与MgCl2的混合物可增强去甲肾上腺素对突触膜中磷脂酶A2水解作用的刺激。在ATP和MgCl2存在的情况下，10μM去甲肾上腺素刺激所需的CaCl2最佳浓度范围为1 - 10μM。在1μM CaCl2存在的情况下，ATP - 2MgCl2的最佳浓度范围为0.1 - 1mM。4. 乙酰胆碱、氨甲酰胆碱、5 - 羟色胺、多巴胺（3,4 - 二羟基苯乙胺）、组胺、γ - 氨基丁酸、谷氨酸和天冬氨酸也刺激突触膜中磷脂酶A2的水解作用。在适当浓度的辅助因子存在下，可获得S形剂量 - 反应曲线，刺激剂浓度在0.1 - 1μM范围内时可获得半数最大刺激。5. 牛磺酸也刺激磷脂酶A2对磷脂酰胆碱的水解作用。甲胺、乙二胺或亚精胺仅有轻微刺激作用。胰高血糖素未产生刺激作用。
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