Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Yangsan 626-870, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:436315. doi: 10.1155/2013/436315. Epub 2013 Jul 23.
Kainic acid (KA) is a neurotoxin that induces epileptic seizures and excitotoxicity in the hippocampus. Acupuncture is frequently used as an alternative therapy for epilepsy, and it has been known to protect hippocampal neurons against KA toxicity. Using proteomic analysis, we investigated protein expression changes in the hippocampus following acupuncture stimulation at HT8. Eight-week-old male C57BL/6 mice (20-25 g) received acupuncture treatment at HT8 acupoint bilaterally once a day for 3 days and were then administered KA (30 mg/kg) intraperitoneally. Twenty-four hours after KA injection, neuronal survival and astrocyte activation in the hippocampus were measured, and protein expression in the hippocampus was identified by 2-dimensional electrophoresis. Acupuncture stimulation at HT8 suppressed KA-induced neuronal death and astrocyte activation in the hippocampus. We identified the changes in the expression of 11 proteins by KA or acupuncture stimulation at HT8 and found that acupuncture stimulation at HT8 normalized the expression of dihydropyrimidinase-related protein 2 and upregulated the expression of transcriptional activator protein pur-alpha, serine/threonine-protein phosphatase 5, and T-complex protein 1 subunit alpha, which are related to the survival of neurons. These results suggest that acupuncture stimulation at HT8 changes protein expression profiles in the hippocampus in favor of neuronal survival in KA-treated mice.
海人酸（KA）是一种神经毒素，可在海马体中引起癫痫发作和兴奋性毒性。针灸经常被用作癫痫的替代疗法，并且已知它可以保护海马神经元免受 KA 毒性的侵害。我们使用蛋白质组学分析方法，研究了针刺 HT8 穴位刺激后海马体中的蛋白质表达变化。将 8 周龄雄性 C57BL/6 小鼠（20-25g）双侧接受 HT8 穴位针刺治疗，每天一次，连续 3 天，然后腹膜内注射 KA（30mg/kg）。KA 注射后 24 小时，测量海马体中的神经元存活和星形胶质细胞激活，并通过 2 维电泳鉴定海马体中的蛋白质表达。针刺 HT8 刺激可抑制 KA 诱导的海马体中的神经元死亡和星形胶质细胞激活。我们通过 KA 或针刺 HT8 刺激鉴定了 11 种蛋白质表达的变化，并发现针刺 HT8 刺激使二氢嘧啶酶相关蛋白 2 的表达正常化，并上调转录激活蛋白 pur-alpha、丝氨酸/苏氨酸蛋白磷酸酶 5 和 T 复合物蛋白 1 亚基 alpha 的表达，这些与神经元的存活有关。这些结果表明，针刺 HT8 刺激改变了 KA 处理小鼠海马体中的蛋白质表达谱，有利于神经元的存活。
